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Physical and Chemical Properties of Lipofuscin 9 D. Significance of Lipofuscin in the Aging Process.. 13 III. MATERIALS AND METHODS l6 A. Canine Brain Specimens l6 B. Porcine Brain Specimens 21 C. Collection and Fixation of Brain • , Specimens 21 D. Processing and Staining Procedures. . 23 E. Visualization of Lipofuscin 27 IV. OBSERVATIONS 31 A. Occurrence and Distribution of Lipofuscin in the Canine Brain. .. . 31 B. Occurrence and Distribution of Lipofuscin in the Porcine Brain.. .. 68 V. DISCUSSION 80 A. Occurrence and Distribution of Lipofuscin in the Canine and Porcine Brain 6o B. Histogenesis of Lipofuscin 84 C.

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