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The ability to design nanowires (NW) in powder technology provides a great advantage for many applications in a variety of fields ranging from medicine to materials science. In this study, nanoparticles were synthesized simultaneously by using an aqueous two‐phase system, which was an environmentally friendly method since the reducing agent that was not spent in the reaction could be effectively recycled and reused. AgNWs (silver nanowires) with numerous high widths and dyes were easily synthesized between two phases using the 5,8‐dibromo‐2‐(butylamino)naphthalene‐1,4‐dione, a 2‐substituted naphthoquinone. Naphthoquinones are known as reducing agents in the production of metal nanoparticles. Synthesized NWs have an average diameter of 90 nm and an average length of 6 μm. The nanowires produced had a round cross section. Characterization of nanowires and nanoparticles was done with UV‐vis, XRD pattern, SEM, and EDX. As a result of characterization, it showed two relatively sharp absorbances in the UV‐vis spectrum at a wavelength of ~358 and 432 nm. In the XRD analysis, five basic peaks attributed to Ag nanoparticles were observed. When SEM images were analyzed, it was determined that Ag nanowires generally have an average diameter of 90 nm and an average length of 6 μm. Anticancer activity test was performed using the MTT test in the HeLa and HT‐29 cell lines. IC50 value was calculated as 11.54 and 16.07 μg ml, and AgNWs showed anticancer properties at high concentration. Evaluating the influence of AgNWs for the first time, this study has indicated that AgNWs exhibit inhibitory properties on the collagenase enzyme. It was observed that the IC50 rate for the enzyme activity was 346.5 mM (r2: 0.955), and Ki value was 41.09 mM.

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The aim of this study was to identify and quantify the phenolic composition of Turanecio hypochionaeus Bosse and determine the anti-urease, anti-lipase, antidiabetic, anti-melanogenesis, antibacterial, and anti-Alzheimer properties. IC 50 results for all enzymes were obtained between 0.234 -116.50 μg/mL and this plant inhibited HMG_CoA R and glucosidase enzymes more with IC 50 values of 0.234 and 116.50 μg/mL, respectively. Among the 11 secondary metabolites identified in T. hypochionaeus extract, chlorogenic acid 255.459 � 1.17 μg g À 1 ), benzoic acid (56.251 � 1.98 μg g À 1 ), and catechin (29.029 � 0.27 μg g À 1 ) were determined as the most abundant phenolic compounds. According to the results of the tested microorganisms, the plant extracts showed antimicrobial and antifungal properties in a dose-dependent manner. In molecular docking study, the interactions of active compounds extracted from Turanecio hypochionaeus plant and showing activity against diverse metabolic enzymes were examined. The most active compound 1, (chlorogenic acid) has À 12.80, À 12.80, À 12.60 and À 12.00 kcal/mol binding energy value against HMG_CoA R, and α-amylase, α-glucosidase, and lipase, respectively.

Single-stranded DNA-binding (SSB) proteins play an important role in DNA metabolism involving DNA replication, recombination, and repair in all living beings. In molecular biology, SSB proteins are used as enhancers to increase the efficiency and specificity of PCR. Thermostable SSB protein eliminates secondary structure or dimer formation and significantly increase the effectiveness of amplification of DNA fragments. In this study, it was ensured that the SSB gene of thermophilic bacteria Thermus aquaticus (T. aquaticus) was cloned into the pET28b vector and expressed in E. coli BL21 (DE3) PLysE cells. Then, the purification of the SSB protein produced in E. coli BL21 (DE3) PLysE cells was performed. 20 mg SSB protein was obtained from 1L bacterial culture, and its purity was more than 90%. It was shown by the PCR experiment that the SSB protein produced in this study could increase the amplification efficiency.