Biosynthesis and characterization of silver nanoparticles from Tricholoma ustale and Agaricus arvensis extracts and investigation of their antimicrobial, cytotoxic, and apoptotic potentials
“…Overproduction of ROS within cells causes lipid peroxidation, protein oxidation, and DNA damage, all of which can trigger the cell's intrinsic apoptotic pathway. When cellular damage is high, the intrinsic apoptotic pathway is triggered by up-regulating pro-apoptotic Bcl-2 family members and down-regulating anti-apoptotic proteins [ 54 , 55 ]. Fig.…”
“…Overproduction of ROS within cells causes lipid peroxidation, protein oxidation, and DNA damage, all of which can trigger the cell's intrinsic apoptotic pathway. When cellular damage is high, the intrinsic apoptotic pathway is triggered by up-regulating pro-apoptotic Bcl-2 family members and down-regulating anti-apoptotic proteins [ 54 , 55 ]. Fig.…”
“…A colorimetric MTT assay determined antiproliferative activity of K‐22 against A549, Saos‐2, MCF‐7, and HT‐29 cell lines as described previously [29,47] . KCN was used as a control compound.…”
Section: Methodsmentioning
confidence: 99%
“…A colorimetric MTT assay determined antiproliferative activity of K-22 against A549, Saos-2, MCF-7, and HT-29 cell lines as described previously. [29,47] KCN was used as a control compound. The cells were exposed to increasing concentrations of (10 ng/ml-0.1562 ng/ ml) and KCN (500 μg/ml-7.81 μg/ml) for 48 h. MTT solution (100 μL) was added to each well at the end of the incubation time and left to incubate for 4 h. DMSO was added to cells to dissolve purple formazan.…”
This study investigated the biological activity of the silver coordination compound K‐22. The IC50 values of K‐22 on cancer cell lines range from 0.797 μg/mL to 3.426 μg/mL, indicating that K‐22 might preferably inhibit A549, Saos‐2, MCF‐7, and HT‐29 cell proliferation and thus have better therapeutic activity. Furthermore, K‐22 stimulated apoptosis via up‐regulation of the mRNA and protein expression level of Bax/Bcl‐2 ratio in A549, Saos‐2, MCF‐7, and HT‐29. K‐22 exhibited antimicrobial activity against S. aureus, E. faecalis, K. pneumonia, P. aeruginosa, C. utilis, and C. albicans. Experimental results show that the compound has inhibitory potential with an IC50 value of 178.10 μM for the BChE (butyrylcholinesterase) enzyme, which has a vital role in the progression of Alzheimer's disease. As a result, compound K‐22 exhibits a strong potential for medical use due to its anticancer, antibacterial, antifungal, and anti‐Alzheimer properties.
“…Due to high throughput and lower cost, microbial synthesis of AgNPs is the most popular. It includes microorganisms, plant leaves, fungi [ 17 ], mushroom [ 18 , 19 ] yeast [ 20 ], and actinomycetes [ 21 ] as reducing agents.…”
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