This study used a combination of chicken manure and chicken broth which was fermented first with the aim to knowing the effect and the best dose of the combination of chicken manure and broth so as to increase the amount of Daphnia magna production optimally. This research is experimental using Completely Randomized Design method which consists of 4 treatments with 5 replications. Data analysis was processed using ANOVA and Duncan. The results showed that the fermentation of manure and chicken broth with a dose of P3 (7.5 mL/L) on day 8 gave the highest yield (41950 ind/L) compared to other treatments. Based on ANOVA analysis, it was shown that the combination treatment of chicken manure and broth compared with no combination on the count of Daphnia magna production had a significant effect (P<0.05). Duncan's test results showed that P3 treatment was significantly different from P0 and P1 but not significantly different from P2 treatment, with an average difference of P2 (5mL/L) 7419 ind/L and P3 (7.5mL/L) 8328 ind/L for 14 days growth.
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