Penelitian ini bertujuan untuk mengisolasi dan menghitung kelimpahan koloni bakteri Vibrio sp. dari air tambak udang dengan 5 lokasi yang berbeda di Kecamatan Simpang Kabupaten Pidie, Aceh. Penelitian ini menggunakan metode eksploratif dan pengambilan sampel menggunakan purposive sampling. Isolasi bakteri menggunakan media selektif TCBSA. Perhitungan kelimpahan koloni Vibrio sp. menggunakan metode Total Plate Count. Hasil penelitian menunjukkan bahwa kelimpahan bakteri Vibrio sp. di Kecamatan Kembang Tanjong untuk kelima kategori lokasi tambak tergolong aman (104 cfu/ml) yaitu 6,2 x 102 – 2,6 x 103 cfu/ml. Berbeda halnya dengan tambak di Simpang Tiga yang masih dapat dikatakan aman hanya tambak K2T2 (2,9 x 103 cfu/ml), K4T2 (2,2 x 104 cfu/ml, K2T1; K5T; K5T2 (2,3 x 104 cfu/ml) karena kelimpahannya 8,34 x 104 cfu/ml, sedangkan bakteri Vibrio lainnya sudah bersifat patogen (105 cfu/ml). Hasil pengukuran kualitas air tambak menunjukkan rata-rata nilai salinitas 10,67-30,33 ppt, suhu 27,9-33,8 oC, pH air 7,65-8,45, DO 5,35-8,42 dan kecerahan 20-55 cm. Tidak adanya hubungan korelasi yang signifikan dari kualitas air tambak terhadap kelimpahan koloni bakteri Vibrio sp.
<p>Mangrove forest is one of the natural resources that has the potential for protection and conservation of nature. Conservation can be defined as a system of management and sustainable use of the environment and natural resources. Tanjung Rejo Village, Percut Sei Tuan sub-district is one of the coastal areas with mangrove forest conditions that have been damaged. This study aims to identify the conservation efforts carried out by the people of Tanjung Rejo Village, precisely in the tourist area of the Mekar Bahari Mangrove Forest. The research was conducted by means of surveys, observations and interviews with the management community. The results show that the conservation efforts carried out by protecting and culturing mangrove plants and making mangrove forests as ecotourism and education of mangrove forests have not getting reached for maximum, if viewed from the presence of mangrove plants and the surrounding environment. Based on surveys and interviews about community perceptions of eco-edutourism development, the community agrees that in the near of future the mangrove forest in Tanjung Sari Village will be used as an eco-edutourism area. It can be said that conservation efforts do not reach the standard, so it is important to develop eco-tourism areas in mangrove forests by increasing public awareness of mangrove preservation.</p><p class="abstrak"> </p><p class="abstrak" align="left"><strong>Keywords:</strong> <em>Conservation, eco-edutourism, Mangrove Forest</em></p>
<p class="StyleAuthorBold">Cilembu Sweet Potato is one type of sweet potato that is typical in the West Java area precisely in the Cilembu village or Sumedang. Cilembu sweet potato has a typical characteristic that is taste like honey if it fermentation and then roasted. Rizospheric bacteria with the ability of phosphate solvents are one of the factors supporting growth in plants. The research aims to identify Rhizosphere bacteria in Cilembu sweet potato roots which have the ability as phosphate solvents at two locations during the growth phase. The study was conducted with qualitative by testing the biochemical characteristics and ability of phosphate solvents. The result of this research show that Rhizosphere bacteria with the ability of phosphate solvents at the location of Cilembu (LCI) and Jatinangor (LJA) are not significantly different. In LCI, 12 isolates were obtained and in LJA 7 isolates were obtained. So that it can be concluded that the diversity of rhizosphere bacteria can be influenced by the characteristics of the soil and the growth phase.</p><p class="StyleAuthorBold"><strong>Keywords</strong>: Cilembu yam, Rizosphere bacteria, phosphate solvent</p>
<p><em>Salak is a fruit that contains various health benefits, including the flesh of the fruit which contains bioactive compounds. Taking bioactive compounds directly from the flesh of the salak fruit will require quite a lot of biomass. Obtaining these bioactive compounds can be streamlined by using endophytic bacteria.</em><em> Endophytic bacteria able to produce compounds of antibacterial. The aimed of this study to examine the antibacterial potential of endophytic bacteria derived from the flesh of the Pondoh and Padangsidempuan salak fruit against pathogenic bacteria, namely Escherichia coli. Method this studies is a laboratory experiment. A total of 10 isolates consisting of 6 isolates of Pondoh salak and 4 isolates of Padangsidempuan salak used were the results of isolation from salak fruit flesh in previous studies. Based on the results of the study, ten isolates indicate of antimicrobial activity to Escherichia coli. The inhibition zone diameter of highest value was owned by SP4 isolate, which was 1.1 mm. These results indicate that endophytic bacteria from the pulp of Pondoh and Padangsidempuan salak possess the potential to produce antibacterial compounds against gram-negative pathogenic bacteria, namely Escherichia coli.</em><em></em></p><p><em> </em></p>
<p class="abstrak">One of the traditional plants used by the community is the kapok plant (<em>Ceiba pentandra</em> L.) apart from being cultivated, parts of the kapok plant are also used as building materials, stuffing for mattresses and traditional medicine. The purpose of this study was to determine the activity of randu leaf extract on the growth of <em>Pseudomonas aeruginosa</em> and <em>Candida albicans</em>. This study used a completely randomized design (CRD) with 6 treatment groups, namely the positive control group with chloramphenicol and ketoconazole. The negative control group used 10% DMSO, and the extract concentration group was 70%, 80%, 90% and 100%. The steps carried out in this study included the extraction of kapok leaves using 96% ethanol as solvent, phytochemical screening and antimicrobial testing using the <em>Kirby-Bauer </em>Disc Diffusion method. Analysis of research data using SPSS 21. From the results of this study, it was found that randu leaf extract contained alkaloids, saponins and tannins. Antimicrobial testing with the kapok leaf extract group resulted in an average inhibition zone of 7.57 mm, 8.0 mm, 8.7 mm, and 9.87 mm against <em>Pseudomonas aeruginosa</em> and 4.67 mm, 5.07 mm, 5.32 mm, and 5.65 mm against <em>Candida albicans</em>. The results of the One Way ANOVA test show that F<sub>table</sub> < F<sub>count</sub> which proves that the leaf extract of Randu provides antimicrobial activity in inhibiting the growth of <em>Pseudomonas aeruginosa</em> and <em>Candida albicans</em>.</p>
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