Exposure to UV light triggers the rapid generation and accumulation of reactive oxygen species (ROS) in skin cells, with consequent increase in oxidative stress and thus in photoaging. Exogenous supplementation with dietary antioxidants and/or skin pretreatment with antioxidant-based lotions before sun exposure might be a winning strategy against age-related skin pathologies. In this context, plants produce many secondary metabolites to protect themselves from UV radiations and these compounds can also protect the skin from photoaging. Phenolic compounds, ascorbic acid and carotenoids, derived from different plant species, are able to protect the skin by preventing UV penetration, reducing inflammation and oxidative stress, and influencing several survival signalling pathways. In this review, we focus our attention on the double role of oxidants in cell metabolism and on environmental and xenobiotic agents involved in skin photoaging. Moreover, we discuss the protective role of dietary antioxidants from fruits and vegetables and report their antiaging properties related to the reduction of oxidative stress pathways.

Solanum lycopersicum represents an important source of antioxidants and other bioactive compounds. Previously two Solanum pennellii introgression lines (IL 7-3 and IL 12-4) were identified as carrying quantitative trait loci (QTL) increasing fruit ascorbic acid and phenolics content. Novel tomato lines were obtained by pyramiding these selected QTLs in the genetic background of the cultivated line M82. Pyramided lines revealed significant increases of total phenolics, phenolic acids, ascorbic acid, and total antioxidant activity compared to parental lines IL 7-3 and IL 12-4 and the cultivated line M82. In addition, tomato extracts obtained from the pyramided lines had no cytotoxic effect on normal human cells while exhibiting a selective cytotoxic effect on aggressive cancer cells. Therefore, the present study demonstrates that it is possible to incorporate favorable wild-species QTLs in the cultivated genetic background to obtain genotypes with higher nutritional value.

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Metabolic control is mediated by the dynamic assemblies and function of multiple redox enzymes. A key element in these assemblies, the P450 oxidoreductase (POR), donates electrons and selectively activates numerous (>50 in humans and >300 in plants) cytochromes P450 (CYPs) controlling metabolism of drugs, steroids and xenobiotics in humans and natural product biosynthesis in plants. The mechanisms underlying POR-mediated CYP metabolism remain poorly understood and to date no ligand binding has been described to regulate the specificity of POR. Here, using a combination of computational modeling and functional assays, we identify ligands that dock on POR and bias its specificity towards CYP redox partners, across mammal and plant kingdom. Single molecule FRET studies reveal ligand binding to alter POR conformational sampling, which results in biased activation of metabolic cascades in whole cell assays. We propose the model of biased metabolism, a mechanism akin to biased signaling of GPCRs, where ligand binding on POR stabilizes different conformational states that are linked to distinct metabolic outcomes. Biased metabolism may allow designing pathway-specific therapeutics or personalized food suppressing undesired, disease-related, metabolic pathways.

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Mammalian cells have enzymes to convert adenosine to inosine by deamination and inosine to hypoxanthine by phosphorolysis, but they do not possess the enzymes necessary to form the free base, adenine, from adenosine. Mycoplasmas grown in broth or in cell cultures can produce adenine from adenosine. This activity was detected in a variety of mycoplasmatales, and the enzyme was shown to be adenosine phosphorylase.Adenosine formation from adenine and ribose 1-phosphate, the reverse reaction of adenine formation from adenosine, was also observed with the mycoplasma en- Mvycoplasma strains related to strain 70-159 were first isolated in our laboratory in 1968. Since then we have isolated over 100 strains, all from cell cultures, which comprise a group distinct from the known mycoplasmas. To our knowledge, members of the 70-159 group have never been isolated from any source other than the cultured cell. illycoplasma Cultures. The agar medium used to isolate mycoplasmas from infected cell cultures and the liquid medium used to propagate mycoplasmas for enzyme assay have been described (8) and are referred to as modified solid medium (Flow no. 5-059) and modified liquid medium .For the production of concentrated mycoplasma suspensions, 0.5 ml. of frozen seed stock was inoculated into 500 ml of modified liquid medium. The cultures were incubated aerobically at 360, with the exception of S. citri, which was incubated anaerobically at 320. The mycoplasma cells were harvested when the medium changed 0.5-1.0 pH unit, as determined by inspection of the phenol red indicator. Previous growth curve studies revealed that the first visually detectable pH change (approximately 0.5 pH unit) coincided with the

The current study provides valuable insights into the health effect of the dietary carotenoids present in fresh and processed tomato fruits. © 2016 Society of Chemical Industry.