Carotenoids in fresh and processed tomato (<i>Solanum lycopersicum</i>) fruits protect cells from oxidative stress injury

Giudice, Rita Del; Petruk, Ganna; Raiola, Assunta; Barone, Amalia; Monti, Daria Maria; Rigano, Maria Manuela

doi:10.1002/jsfa.7910

Cited by 43 publications

(31 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Human A431, murine BALB/c‐3T3, and SVT2 fibroblasts were obtained from ATCC and were maintained in culture as previously described . For dose–response experiments, cells were seeded in 96‐well plates at a density of 2.5×10 3 cells per well; 24 h after seeding, increasing amounts of DiRu‐1‐encapsulated AFt (0.15–3 μ m ) or DiRu‐1 (0.05–0.2 μ m ) were added to the cells for 48 h. Cell viability was then assessed by MTT [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] assay, as described by Ferraro et al .…”

Section: Methodsmentioning

confidence: 99%

“…[13] Cell culture and cell growth inhibition Human A431, murine BALB/c-3T3, and SVT2 fibroblasts were obtained from ATCC and were maintained in culture as previously described. [62] For dose-response experiments, cells were seeded in 96-well plates at ad ensity of 2.5 10 3 cells per well;2 4h after seeding, increasing amounts of DiRu-1-encapsulated AFt (0.15-3 mm)o rD iRu-1 (0.05-0.2 mm)w ere added to the cells for 48 h. Cell viability was then assessed by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide] assay, [48] as described by Ferraro et al [6] Cell survival was expressed as the percentage of viable cells in the presence of test compound relative to controls. Twog roups of cells were used as controls:u ntreated cells and cells supplemented with identical volumes of buffer.E ach sample was tested in three independent analyses, each carried out in triplicate.…”

Section: Full Papers Experimental Sectionmentioning

confidence: 99%

See 1 more Smart Citation

Encapsulation of the Dinuclear Trithiolato‐Bridged Arene Ruthenium Complex Diruthenium‐1 in an Apoferritin Nanocage: Structure and Cytotoxicity

et al. 2019

Self Cite

View full text Add to dashboard Cite

The effects of encapsulating the cytotoxic dinuclear trithiolato‐bridged arene ruthenium complex [(η6‐p‐MeC6H4iPr)2Ru2(μ2‐S‐p‐C6H4tBu)3]Cl (DiRu‐1) within the apoferritin (AFt) nanocage were investigated. The DiRu‐1‐AFt nanocarrier was characterized by UV/Vis spectroscopy, ICP‐MS, CD and X‐ray crystallography. In contrast to previously reported Au‐ and Pt‐based drug‐loaded AFt carriers, we found no evidence of direct interactions between DiRu‐1 and AFt. DiRu‐1‐AFt is cytotoxic toward immortalized murine BALB/c‐3T3 fibroblasts transformed with SV40 virus (SVT2) and human epidermoid carcinoma A431 malignant cells, and exhibits moderate selectivity for these cancer cells over normal BALB/c‐3T3 cells. DiRu‐1‐AFt triggers the production of reactive oxygen species, depolarization of mitochondrial membrane potential, and induces cell death via p53‐mediated apoptosis. Comparison between our data and previous results suggests that the presence of specific interactions between a metal‐based drug and AFt within the protein cage is not essential for drug encapsulation.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Full Papers Experimental Sectionmentioning

confidence: 99%

Encapsulation of the Dinuclear Trithiolato‐Bridged Arene Ruthenium Complex Diruthenium‐1 in an Apoferritin Nanocage: Structure and Cytotoxicity

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Tomato (Solanum lycopersicum L.) is one of the most consumed vegetables worldwide also owing to the development of products such as soups, juices, purees, and sauces [1]. Tomato is an essential component of the Mediterranean diet and of other traditional diets.…”

Section: Introductionmentioning

confidence: 99%

The Use of a Plant-Based Biostimulant Improves Plant Performances and Fruit Quality in Tomato Plants Grown at Elevated Temperatures

et al. 2020

Self Cite

View full text Add to dashboard Cite

Abiotic stresses can cause a substantial decline in fruit quality due to negative impacts on plant growth, physiology and reproduction. The objective of this study was to verify if the use of a biostimulant based on plant and yeast extracts, rich in amino acids and that contains microelements (boron, zinc and manganese) can ensure good crop yield and quality in tomato plants grown at elevated temperatures (up to 42 °C). We investigated physiological responses of four different tomato landraces that were cultivated under plastic tunnel and treated with the biostimulant CycoFlow. The application of the biostimulant stimulated growth (plants up to 48.5% taller) and number of fruits (up to 105.3%). In plants treated with the biostimulant, antioxidants contents were higher compared to non-treated plants, both in leaves and in fruits. In particular, the content of ascorbic acid increased after treatments with CycoFlow. For almost all the traits studied, the effect of the biostimulant depended on the genotype it was applied on. Altogether, the use of the biostimulant on tomato plants led to better plant performances at elevated temperatures, that could be attributed also to a stronger antioxidant defence system, and to a better fruit nutritional quality.

show abstract

“…HaCaT cells were seeded at a density of 3.5 × 10 4 cells/cm 2 (100 mm eukaryotic cell plates) in complete medium for 24 h and then treated with 10 µg/mL of MeDHICA-melanin for 120 min. After UVA irradiation, cells were kept at 37 • C for 90 min, before performing the thiobarbituric acid reactive substances (TBARS) assay as described [48]. Briefly, cells were detached and 5 × 10 4 cells suspended in 0.67% TBA containing 20% trichloroacetic acid (TCA) (1:1 v/v).…”

Section: Analysis Of Lipid Peroxidation Levelsmentioning

confidence: 99%

A Melanin-Related Phenolic Polymer with Potent Photoprotective and Antioxidant Activities for Dermo-Cosmetic Applications

Liberti¹,

Alfieri²,

Monti³

et al. 2020

Antioxidants

Self Cite

View full text Add to dashboard Cite

Eumelanins, the dark variant of skin pigments, are endowed with a remarkable antioxidant activity and well-recognized photoprotective properties that have been ascribed to pigment components derived from the biosynthetic precursor 5,6-dihydroxyindole-2-carboxylic acid (DHICA). Herein, we report the protective effect of a polymer obtained starting from the methyl ester of DHICA (MeDHICA-melanin) against Ultraviolet A (UVA)-induced oxidative stress in immortalized human keratinocytes (HaCaT). MeDHICA-melanin was prepared by aerial oxidation of MeDHICA. At concentrations as low as 10 µg/mL, MeDHICA-melanin prevented reactive oxygen species accumulation and partially reduced glutathione oxidation in UVA-irradiated keratinocytes. Western blot experiments revealed that the polymer is able to induce the translocation of nuclear factor erythroid 2–related factor 2 (Nrf-2) to the nucleus with the activation of the transcription of antioxidant enzymes, such as heme-oxygenase 1. Spectrophotometric and HPLC analysis of cell lysate allowed to conclude that a significant fraction (ca. 7%), consisting mainly of the 4,4′-dimer of MeDHICA (ca. 2 μM), was internalized in the cells. Overall these data point to the potential use of MeDHICA-melanin as an antioxidant for the treatment of skin damage, photoaging and skin cancers.

show abstract

Carotenoids in fresh and processed tomato (Solanum lycopersicum) fruits protect cells from oxidative stress injury

Abstract: The current study provides valuable insights into the health effect of the dietary carotenoids present in fresh and processed tomato fruits. © 2016 Society of Chemical Industry.

Cited by 43 publications

References 37 publications

Encapsulation of the Dinuclear Trithiolato‐Bridged Arene Ruthenium Complex Diruthenium‐1 in an Apoferritin Nanocage: Structure and Cytotoxicity

Encapsulation of the Dinuclear Trithiolato‐Bridged Arene Ruthenium Complex Diruthenium‐1 in an Apoferritin Nanocage: Structure and Cytotoxicity

The Use of a Plant-Based Biostimulant Improves Plant Performances and Fruit Quality in Tomato Plants Grown at Elevated Temperatures

A Melanin-Related Phenolic Polymer with Potent Photoprotective and Antioxidant Activities for Dermo-Cosmetic Applications

Contact Info

Product

Resources

About