No abstract

Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan, sitotoksisitas dan kandungan fukosantin ekstrak etil asetat beberapa jenis rumput laut coklat. Aktivitas antioksidan dianalisis dengan menggunakan metode 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) sedangkan aktivitas sitotoksik diuji dengan menggunakan metode {3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide} (MTT). Kadar fukosantin dianalisis menggunakan metode Kromatografi Cair Kinerja Tinggi (KCKT). Enam jenis rumput laut coklat yaitu Sargassum ilicifolium, Sargassum binderi, Turbinaria decurrens, Turbinaria ornata, Padina australis dan Hormophysa triquetra telah dikoleksi dari pantai Binuangeun, Propinsi Banten. Ekstrak P. australis menunjukkan aktivitas antioksidan yang paling tinggi diiikuti oleh H. triquetra dan T. decurrens. Hasil uji memperlihatkan bahwa ekstrak T. decurrens menghambat pertumbuhan sel HeLa dan T47D sedangkan H. triquetra hanya menghambat pertumbuhan sel T47D. Kadar fukosantin tertinggi dihasilkan oleh ekstrak H. triquetra sebesar 88,5 mg/g diikuti oleh T. decurrens, dan P. australis, berturut-turut sebesar 86,9 mg/g, dan 77,8 mg/g. Di lain sisi, kandungan fukosantin ekstrak S. ilicifolium, T. ornata, dan S. binderi relatif rendah (<20 ppm).

Indonesia has a great sea cucumber resource which is useful as functional food. Even though much information about the biopotency of sea cucumbers have been published, however, studies on nutrition value of Indonesian sea cucumbers are still beneficial due to the great diversity and uniqueness of each species. The present work was intended to obtain information about chemical composition and fatty acid profile of 4 (four) types of sea cucumbers which are frequently found in Halmahera water, North Maluku (Molucca), one of sea cucumbers producing area in Indonesia. The samples coded as H-03, H-04, H-05 and H-10, were identified as Bohadschia argus, Holothuria fuscogilva, Thelenota ananas, and Actinophyga lecanora. All sea cucumbers showed high protein content, more than 60% (drybase/db), except for T. ananas which had the lowest protein content (48.26% db). As for fats, T. ananas showed the highest amount (2.35%db) with dominated saturated fatty acids (SFA). On the other side, A. lecanora showed higher value of PUFA compared to SFA and MUFA. Palmitic acid (C16:0) was the most abundant SFA in the most samples with the amount of 0.49-4.9 mg/g sample. Among PUFA, eicosapentaenoic acid/EPA (C20:5n3) was detected, and eicosatrienoic acid (C20:3n6) was relatively higher than the others. The ratio of 6/3 fatty acid was 1.2-3.2 showing that total 6 fatty acid was higher than total 3 fatty acid. However, this value is safe enough to protect against chronic and degenerative diseases.

Increasing energy needs cause diminishing energy resources. This encourages the searchfor renewable energy sources to anticipate scarcity. One of the new energy source is microalgae.Microalgae have a high variation of species and have a great potential to be developed as foodand other chemical products. Microalgae has been developed as a potential source of biodieselto replace petroleum fuels derived from foss ils. Of several microalgae s pecies studied,Botryococcus braunii produces the largest oil content, i.e. 75% dry weight. This paper describessteps of producing oil from B. brauniiwhich includes preparation of microalgae biomass,biomass harvesting, and extraction of oil. Oil content of B. braunii is composed mostly ofhydrocarbons (± 15–76% by dry weight), called botryococcene. This type of hydrocarbon ispotential as an energy source of biodiesel.

Nutraseutikal berbasis Fish Serum Albumin (FSA) adalah produk komersial yang berasal dari sumber perairan. Namun, oleh karena produk ini secara umum diproduksi dari ikan gabus (Channa striata) di alam, pasokan bahan baku dapat menjadi masalah pada produksi yang berkesinambungan. Penelitian ini telah dilakukan dengan menganalisis kandungan FSA dari 17 ikan air tawar budidaya, untuk mendapatkan bahan baku alternatif bagi produk nutraseutikal berbasis FSA. 3-10 individu ikan air tawar (150-500 g) dari jenis ordo Perciformes, Anguilliformes, Cypriniformes, Osteoglossiformes, dan Siluriformes telah diambil secara acak dari lokasi budidaya ikan di Bogor dan Cianjur (Jawa Barat). Ekstraksi protein larut air dilakukan menggunakan Ultra Turax homogeniser dengan pelarut akuabides. Analisis FSA telah dilakukan menggunakan High Performance Liquid Chromatography (HPLC). Hasil dari penelitian menunjukkan bahwa kadar FSA dari 17 ekstrak air dari ikan berada pada rentang 42,51 to 215,57 mg/g, dengan kadar FSAikan gabus pembanding adalah 107,28 ± 3,2 mg/g. Konsentrasi FSA tertinggi ditemukan sebesar 215,57 ± 52,84 mg/g dari ekstrak air ikan gurame (Osphronemus gouramy). Analisis lebih lanjut terhadap komposisi asam amino menggunakan Gas Chromatography-Flame Ionization Detector (GC-FID) menemukan bahwa ekstrak air ikan gabus memiliki konsentrasi asam amino esensial dan non-esensial yang lebih tinggi dibandingkan dengan ikan gurame. Hal ini menunjukkan banyaknya protein lain selain FSA pada ekstrak air ikan gabus dibandingkan ikan gurame. Berdasarkan hasil ini, ekstrak air ikan gurame memiliki konsentrasi FSA yang lebih besar dan relatif lebih murni dibandingkan ekstrak air ikan gabus, sehingga merupakan alternatif yang prospektif sebagai bahan baku untuk produk nutraseutikal berbasis FSA. KATA KUNCI: serum albumin ikan, nutrasetikal, budidaya, ikan air tawar ABSTRACT Fish Serum Albumin (FSA) based nutraceutical is a commercial product from aquatic resources. However, as the product is usually developed from wild freshwater snakehead fish (Channa striata), raw material supply may become a problem at continuous production. This research had been done to analyze FSA level in 17 aquaculture freshwater fishes, aimed to find an alternative of raw material sources in FSA based neutrasetical products. 3-10 individuals of freshwater fishes (150-500 g) from Perciformes, Anguilliformes, Cypriniformes, Osteoglossiformes, and Siluriformes ordo were taken randomly from Bogor and Cianjur (West Java) aquaculture fields. Aqueous protein extraction was performed by Ultra Turax homogeniser with aquabidest solvent. Analysis of albumin content had been done by High Performance Liquid Chromatography (HPLC). The results showed that FSA level in aqueous extracts of observed fishes were 42.51 to 215.57 mg/g, while FSA in aqueous snakehead fish extract was 107.28 ± 3.2 mg/g. The highest FSA concentration was 215,57 ± 52,84 mg/g from giant gouramy (Osphronemus gouramy). Further analysis at amino acid composition by Gas Chromatography-Flame Ionization Detect...

Fish protein hydrolysates (FPH) which contains mixture of small protein or peptide and free amino acids may be good to be applied in children’s diet, preventing and combating malnutrition problem through readily absorbed essential amino acid. Malnutrition is still a big issue in East Asian countries including Indonesia. According to FAO, in 2005—2015, the percentage of children under five who experience stunting increased from 28.6% to 36.4%, while the percentage of stunting for Indonesian children was 37.2% (2013), 35.6% (2010), 36.8% (2007). Malnutrition in children can lead to serious problems such as abnormal brain development and susceptibility to various infectious diseases. The last report in 2016 revealed that stunted children have lower content of essential amino acid in their serum compared to the normal children. The aims of this study were to produce and analyze the amino acid profile of two fish hydrolysate protein (FPH) products from Sardinella using local microbial protease isolated from hot marine water. The FPH-1 and FPH-2, both from soluble and solid part of hydrolyzed fish were dominated by amino acid lysine and leucine as well as glutamic acid. Both FPH products can potentially be used as ingredients for developing food for malnourished children.

Study on chemical content of swamp fishes from Merauke has been conducted to obtain nutritional status  of these fishes. Sampling was conducted  twice (August and November 2015) in Merauke. There were six dominant fish species, namely striped snakehead (Channa striata), nile tilapia (Oreochromis niloticus), tade gray mullet (Liza tade), philippine catfish (Clarias batrachus), barramundi (Lates calcarifer), and climbing perch (Anabas testudineus). Result on proximate analysis showed that the fishes had moisture content of 75.73±0.25%-81.45±0.04%, ash 0.94±0.01%-1.26±0.21%, protein 17.11±0.09%-18.92±0.05%, and lipid 0.59±0.19%-3.80±0.63%. The dominant essential amino acid of 6 swamp fishes from Merauke was lysine followed by leucine. Meanwhile the dominant non-essential amino acid was glutamic acid (32.26+0.61 mg/g), followed by aspartic acid. Calcium (Ca) was the dominant macro mineral for all fishes studied, being the highest was in nile tilapia (328.76±8.14 mg/100 g). Micro mineral was dominated by the presence of Selenium (Se) which the highest was in nile tilapia (0.084±0.005 mg/100g). These fishes were also rich in albumin, being the highest was stripe snakehead (138.59±1.68 mg/g). The average of total fatty acid showed that the fishes had saturated fatty acid (SFA) content of 46.30±0.27%, monosaturated fatty acid (MUFA) 9.20±1.6%  and polyunsaturate fatty acid (PUFA) 34.49±0.44% of total fatty acid. From the result, it can be concluded that fish harvested from swamp of Merauke and surrounding area had a good nutritional value.

Indonesia is rich of giant fresh water prawn (GFP) germ plasms. Best utilization of these resources for the purpose of either aquaculture development or conservation of genetic resources requires some information on the structure and levels of their genetic diversity. This study was aimed to characterize those GFP genetic resources by applying RAPD genetic markers. Six Indonesian populations of GFP from Asahan, Barito, Ciasem, Ogan, GImacro and Papua were collected and analyzed for their genetic variation using five RAPD primers. The results showed the diversity within the populations, as revealed by the level of polymorphism, ranged from 29% to 76% while genetic divergence between populations as shown by genetic distance ranged from 0.04 to 0.50. In terms of genetic divergence, two genetically distinct groups of GFP, namely the Papua GFP in one group and the remaining five GFP populations in the other, were identified. The results also showed the presence of specific population markers that are useful for genetic identification of GFP populations. Implication of these finding with regard to breed development is discussed.