Filariasis is a chronic infectious disease, caused by filarial worms in the lymph and lymph nodes cause lymphangitis and elephantiasis. Batuah Village is the area in Sampit with the largest percentage of microfilariae compared to the surrounding area. From 196 the number of Batuah villagers examined by the filariasis elimination officer of Kotawaringin Timur District Health Office in 2013 obtained 5 positive cases of filariasis with 2.55% microfilaria number which is dominated by filaria worm Brugia malayi. From the local health center has also conducted a mass drug treatment program with DEC medication, combined with albendazole for 12 days on 14-16 July 2014. The aim of this study was to determine the success rate of treatment conducted by the health officer against filariasis patients in the Batuah village. The method used in this microfilariae examination is a microscopic method. Capillary blood was taken on 4 filarial patients who tested positive on the previous examination who had been given DEC + Albendazole treatment, the result from 4 samples that examined 1 positive sample of 4 patients. The implementation of medication that has been done by public health office was successful because there was a decrease of microfilaria rate which was previously 2.55% to 0,51% (<1%).
<span lang="EN-US">Early detection of </span><span lang="IN">Soil-Transmitted Helminths (STHs)</span><span lang="IN"> infection</span><span lang="EN-US"> in laboratory </span><span lang="IN">medical facilities </span><span lang="EN-US">is one of the government's efforts in </span><span lang="IN">its elimination</span><span lang="EN-US">. Government regulations recommend Kato</span><span lang="IN">-</span><span lang="EN-US">Katz's </span><span lang="IN">quantitative </span><span lang="EN-US">method </span><span lang="IN">for</span><span lang="IN">the diagnosis </span><span lang="EN-US">of </span><span lang="IN">STHs infection</span><span lang="EN-US">. Kato</span><span lang="IN">-</span><span lang="EN-US">Katz's domesti</span><span lang="IN">c product</span><span lang="EN-US"> is not available</span><span lang="EN-US">yet</span><span lang="IN">.</span><span lang="EN-US"> Th</span><span lang="IN">is</span><span lang="EN-US"> research aims to create a laboratory-scale</span><span lang="IN"> modified</span><span lang="EN-US"> Kato</span><span lang="IN">-</span><span lang="EN-US">Katz kit for early detection of</span><span lang="IN"> STHs</span><span lang="IN">infection by fecal egg counts (FECs) in estimating the number of eggs per gram of stool (EPG).</span><span lang="IN">An experimental study was conducted to create a resin template, spatulas, and 3M-adhesive tape as a modified Kato-Katz Kit. 120 </span><span lang="EN-US">Kato-Katz thick smear preparations were done</span><span lang="IN"> from 30 samples for modified and standard Kato-Katz with duplication. The</span><span lang="EN-US"> amount of stool for each</span><span lang="IN"> was weighed. All Kato-Katz thick smear microscopic examination was done at least 60 minutes by identifying the STHs eggs and counting them.</span><span lang="EN-US">The result</span><span lang="IN"> for both methods</span><span lang="EN-US"> showed</span><span lang="IN"> no differences in EPG for <em>Trichuris trichiura </em>eggs p-value=0.012<em> </em>(p<0.05),<em> </em>meanwhile the differences for <em>Ascaris lumbricoides eggs </em>p-value=0.156<em>,</em> and Hookworm eggs p-value=0.102 (p>0.05). Under microscopic examination, some <em>A.lumbricoides </em>eggs morphology was not visible because the adhesive in modified Kato-Katz blurry it, and the Hookworm fragile eggshells made it disappear within less than 60 minutes.</span><span lang="EN-US">The study concluded</span><span lang="IN"> no difference in EPG for <em>T.trichiura </em>eggs by FECs between</span><span lang="IN">modified</span><span lang="EN-US"> Kato-Katz</span><span lang="IN">as this study product with the standard. It is recommended to improve modified Kato-Katz in further study, especially the adhesive tape, before using it as a quantitative tool</span><span lang="IN">for</span><span lang="IN">early </span><span lang="EN-US">detection of </span><span lang="IN">STHs infection.</span><p> </p>
Soil-Transmitted Helminths (STH) infections are the most widespread neglected tropical diseases, primarily affecting morbidity in School Age Children (SAC). STH infection in SAC remains a public health problem in Martapura River Banks. Early and accurate STH detection is essential to determine an effective treatment for reducing morbidity. The Health Department recommends detecting STH infection by Kato Katz (KK) but in public health centers only by the direct method. This research objective is to confirm STH infection prevalence by Kato-Katz (KK) in elementary school children in West Martapura River Banks, South Kalimantan, Indonesia, against the direct method. The modified KK templates were developed from acrylic material. A total number of 253 children 7-12 years old in four West Martapura elementary schools were examined for STHs eggs using both microscopies modified KK against direct method by two trained laboratory technicians. STHs infection prevalence was 50 (19.76%), interpreted as low prevalence by direct and 78 (30.83%), moderate by KK. The concordance between both methods was significantly based on Cohen’s kappa (?=0.682). Even though the concordance of both methods was strong, the prevalence of STHs by modified KK was 30.08% higher than by Direct, which was only 20.33% positive. This study recommended using the KK for STHs diagnostic for prevalence measurement in low STHs infection.
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