The major protein component present in the capsule and thread of a sea anemone nematocyst consists of monomers of a collagen-like protein linked by disulfide bonds. Purified nematocyst capsules and threads are rich in hydroxyproline, and dissolved by disulfide reducing agents. Electrophoresis of the dissolved component on acrylamide gel results in a single detectable protein band.
Journal of Liquid ChromatographyPublication details, including instructions for authors and subscription information: ABSTRACT The e f f e c t o f column l e n g t h , s t a t i o n a r y phase chemistry, f l o w r a t e and g r a d i e n t v a r i a t i o n and d i r e c t i o n on t h e s e p a r a t i o n o f seven p r o t e i n s was evaluated. I n general, r e s o l u t i o n increased with s h o r t e r columns and h i g h e r f l o w r a t e s . N o n t r a d i t i o n a l r e v e r s e g r a d i e n t s (from a c e t o n i t r i l e t o b u f f e r ) sometimes produced b e t t e r p r o t e i n separations than t r a d i t i o n a l reversed phase g r a d i e n t s . Bonded phase c h e m i s t r y has l i t t l e e f f e c t on g r a d i e n t 1934 BLANQUET, BUI, AND ARMSTRONG p r o t e i n separations, b u t s i l i c a g e l p r o p e r t i e s (e.g., s u r f a c e area, p o r e s i z e , e t c . ) d i d . Both r e t e n t i o n and r e s o l u t i o n v a r i e d considerably w i t h t h e n a t u r e o f t h e g r a d i e n t used.
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