A B S T R A C T Plasma acetate turnover and oxidation were determined in 11 healthy subjects by the constant infusion of a trace amount of [1_-4C]acetate for 6 h. The subjects ages ranged from 22 to 57 yr. There was a positive correlation (P < 0.001) between plasma acetate concentration and turnover rate, and a negative correlation (P < 0.001) between turnover and age. The plasma acetate concentration in the subjects 22-28 yr old was 0.17 vs. 0.13 mM (P < 0.02) in subjects 40-57 yr old. The plasma acetate turnover rate was also greater in the younger age group (8.23+0.66 vs. 4.98+0.64 ,umol/ min -kg, P < 0.01). Approximately 90% of the plasma acetate turnover was immediately oxidized to CO2 in both age groups, however, 13.2±+0.89% of the CO2 output in the younger group was derived from plasma acetate oxidation compared to 7.9+0.94% in the older group (P < 0.01). The mean plasma acetate concentration, turnover, and oxidation in six cancer patients 47-63 yr old were similar to the values observed in the age-matched healthy subjects.
Mycobacterium fortuitum utilizes certain stereoisometric mixtures of individual multimethyl branched alkanes as sole carbon source, including 2,6(R), 10(S), 14(RS)-tetramethylhexadecane; 2.6(R), 10(S), 14(RS)-tetramethylheptadecane; 2,6(RS), 10(RS)-trimethyltetradecane, and 2,6(R), 10(S)-trimethylpentadecane. Products of oxidation isolated from the bacterial lipids were acids derived predominantly from oxidation of the isopropyl terminus of each alkane, except in the case of 2,6(RS), 10(RS)-trimethyltetradecane. With the latter, acids from oxidation at either terminus were detected in comparable proportions.
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