A domainal pattern of top to the NNW and top to the SSE shear senses is indicated by both microstructures and c-axis and a-axis fabrics within the plastically deformed and dynamically recrystallized quartz veins located in the gneissic border facies around the western part of the pluton (Fig. DR.1). A top to the SSE shear sense is dominant on the southern margin of the pluton (i.e. pluton up shear sense), whereas on the northern margin km-scale domains of top to the SSE and top to the NNW (i.e. pluton up shear sense), are recorded. Domains of symmetric fabrics, indicating approximate coaxial deformation conditions, are recorded on both the northern and southern pluton margins. In between the margins, plastically deformed quartz veins located along the pluton long axis mostly exhibit symmetric fabrics.
This manuscript describes the production of Molecularly Imprinted Polymer nanoparticles (nanoMIPs) for the cardiac biomarkers heart-fatty acid binding protein (H-FABP) and ST2 by solid-phase synthesis, and their use as synthetic antibodies in a multiplexed sensing platform. Analysis by Surface Plasmon Resonance (SPR) shows that the affinity of the nanoMIPs is similar to that of commercially available antibodies. The particles are coated onto the surface of thermocouples and inserted into 3D-printed flow cells of different multiplexed designs. We demonstrate it is possible to selectively detect both cardiac biomarkers within the physiologically relevant range. Furthermore, the developed sensor platform is the first example of a multiplex format of this thermal analysis technique which enables simultaneous measurements of two different compounds with minimal cross selectivity. The format where three thermocouples are positioned in parallel exhibits the highest sensitivity, which is explained by modelling the heat flow distribution within the flow cell. This design is used in further experiments and proof-of-application of the sensor platform is provided by measuring spiked fetal bovine serum samples. Due to the high selectivity, short measurement time, and low-cost of this array format, it provides an interesting alternative to traditional immunoassays. The use of nanoMIPs enables a multi-marker strategy, which has the potential to contribute to sustainable healthcare by improving reliability of cardiac biomarker testing.
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