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of aerosolized pathogenic fungi on laboratory surfaces. I. Tissue phase. Appl. Microbiol. 11:436-445. 1963.-The effect of several fungicides on laboratory surfaces contaminated with the tissue phase of aerosolized Blastomyces dermatitidis, Coccidioides immitis, Cryptococcus neoformans, and Histoplasma capsulatum was ascertained. A statistical analysis of the data shows the correlation between fungi, surfaces, time, and concentration of disinfectant. All fungicides were effective at established times and concentrations, and the type of contaminated surface affected the fungicidal efficacy. By interpolating plotted graphs, laboratory personnel may determine, with a given fungicide, the concentration and time required to disinfect instruments, pipettes, gloves, bench tops, and floors contaminated with a tissue phase of pathogenic fungi. Review of the literature has revealed no report on the efficacy of disinfectants for laboratory surfaces contaminated with aerosolized pathogenic fungi such as Blastomyces, Coccidioides, Cryptococcus, and Histoplasma. There seems to be no universally accepted test for fungal disinfectants. The first well-known method of testing germicides employed silk threads impregnated with spores of Bacillus anthracis (Koch, 1881). An improvement was made by Rideal and Walker (1903). It and other modifications are still in use today. The phenol coefficient test is recommended, but is not an official test of the Food and Drug Administration. Phillips (1960) stated that the phenol co

KRUsE, RICHARD H. (U.S. Arm-n 13iological Laboiratories, Fort I)etrick, Freder-ick,-id.), 1THERON D. GREEN, RICHARI) C. CHAMBERS, AND AMARIAN AX\. JONES. Disinfection of aerosolized pathogenic fungi on laboratory surfaces. II. Culture phase. Appl.-Microbiol. 12: 155-160. 1964.-The effect of several fuingicides on laboratory surfaces contaminated with the culture (spore) p)hase of aerosolized Blastomyces dermatitidis, Coccidioides

The effect of several fungicides on laboratory surfaces contaminated with the tissue phase of aerosolized Blastomyces dermatitidis, Coccidioides immitis, Cryptococcus neoformans , and Histoplasma capsulatum was ascertained. A statistical analysis of the data shows the correlation between fungi, surfaces, time, and concentration of disinfectant. All fungicides were effective at established times and concentrations, and the type of contaminated surface affected the fungicidal efficacy. By interpolating plotted graphs, laboratory personnel may determine, with a given fungicide, the concentration and time required to disinfect instruments, pipettes, gloves, bench tops, and floors contaminated with a tissue phase of pathogenic fungi.

The effect of several fungicides on laboratory surfaces contaminated with the culture (spore) phase of aerosolized Blastomyces dermatitidis, Coccidioides immitis , and Histoplasma capsulatum was ascertained. The culture (spore) phase was more resistant to the action of the fungicides than was the tissue (yeast) phase. The addition of a wetting agent increased the efficiency of several fungicides. The time required for disinfection with a given concentration of fungicide, or the concentration required to disinfect within a given time, can be determined by interpolating the plotted graphs.