Disinfection of Aerosolized Pathogenic Fungi on Laboratory Surfaces. 2. Culture Phase

Kruse, Richard H.; Green, Theron D.; Chambers, Richard C.; Jones, Marian W.

doi:10.21236/ad0424667

Cited by 10 publications

(20 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has also been shown that Histoplasma capsulatum, Blastomyces dermatitidis and Coccidioides immitis aerosolized onto a surface in the tissue (yeast) phase are killed in less than 1 min by 70% ethanol, but this requires up to 20 min when in the culture (spore) phase. 8 However, there is extensive evidence that adherent bacteria are significantly more resistant to antimicrobial agents than free organisms. Susceptibility tests with in vitro biofilm models have shown survival of bacterial biofilms after treatment with antibiotics at concentrations hundreds of times higher than the minimum inhibitory concentration of bacteria measured in suspension culture.…”

Section: Discussionmentioning

confidence: 99%

Ethanol disinfection of plastic-adherent micro-organisms

Chambers

Peddie

Pithie

2006

Journal of Hospital Infection

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Ethanol disinfection of plastic-adherent micro-organisms

Chambers

Peddie

Pithie

2006

Journal of Hospital Infection

View full text Add to dashboard Cite

“…(Stevens et al, 2009). Two older publications (Kruse et al, 1963, 1964) investigated the disinfection of laboratory surfaces exposed to aerosolized “tissue” (i.e., spherule) (Kruse et al, 1963) and “culture” (i.e., arthroconidia) (Kruse et al, 1964) phases of four and three, respectively, different pathogenic fungi, including C. immitis . These publications suggest that a number of disinfectants are effective against Coccidioides spp., given sufficient concentration and contact time, including ethanol, sodium hypochlorite, formaldehyde, phenolics, peracetic acid, an iodophor, and a quaternary ammonium compound.…”

Section: Introductionmentioning

confidence: 99%

Effective Disinfectants forCoccidioides ImmitisandC. posadasii

et al. 2015

View full text Add to dashboard Cite

The lack of published data on effective disinfectants and contact times for use on the fungal pathogens Coccidioides immitis and C. posadasii prompted the authors to investigate the fungicidal activity of three commonly used laboratory disinfectants on arthroconidia harvested from C. immitis strain 2009. They tested the ability of 10% bleach, 70% ethanol, and Vesphene® IIse to inactivate 107 arthroconidia in an aqueous suspension within 1, 2, 5, 10, or 20 minutes of contact time. Both 10% bleach and 70% ethanol provided a 7-log10 reduction in arthroconidia in less than 1 minute, with no growth observed at any of the tested time points. Vesphene® IIse was less effective, providing a 6-log10 reduction in arthroconidia after 5 minutes, but was unable to completely inactivate all of the arthroconidia, even after 20 minutes of contact time.

show abstract

“…THE TRANSITION YEARS: 1957-1963 The biological safety conferences during this period continued to be sponsored by and held at the three collaborating U.S. Army BW laboratories. The conference agendas, however, were planned to include non-classified sessions to enable the broader sharing of biological safety information with safety personnel not associated with the U.S. Army BW programs.…”

Section: Rd Biological Safety Conferencementioning

confidence: 99%

A History of the American Biological Safety Association Part I: The First Ten Biological Safety Conferences 1955–1965

Barbeito

Kruse²

1997

Journal of the American Biological Safety Association

Self Cite

View full text Add to dashboard Cite

were discussing how to manage better the extensive correspondence and telephone conversations on safety issues among the three BW laboratories, the idea of a conference emerged as a collective thought. The idea was quickly ratified by the safety directors at the three BW installations as the perfect vehicle for sharing safety information. Due to the nature of the work conducted at the BW laboratories, papers presented at the conference would have to be cleared in advance by security officers and attendance would be restricted to persons with secret clearances. These restrictions were considered at the time a small burden in contrast to the enormous benefit that would be gained from sharing common experiences and collaboratively reolving important safety problems.

show abstract

Disinfection of Aerosolized Pathogenic Fungi on Laboratory Surfaces. 2. Culture Phase

Cited by 10 publications

References 0 publications

Ethanol disinfection of plastic-adherent micro-organisms

Ethanol disinfection of plastic-adherent micro-organisms

Effective Disinfectants forCoccidioides ImmitisandC. posadasii

A History of the American Biological Safety Association Part I: The First Ten Biological Safety Conferences 1955–1965

Contact Info

Product

Resources

About