This study aimed to verify the in vitro ability of beer
fermentation residue (BFR) containing Saccharomyces cerevisiae
cells and five commercial products that differed in the viability and integrity
of S. cerevisiae cells to remove aflatoxin B1
(AFB1) from a citrate-phosphate buffer solution (CPBS). BFR was
collected at a microbrewery and prepared by drying and milling. The commercial
yeast-based products were as follows: inactive intact yeast cells from beer
alcoholic fermentation, inactive intact yeast cells from sugarcane alcoholic
fermentation, hydrolyzed yeast cells, yeast cell walls and active yeast cells.
Adsorption assays were performed in CPBS spiked with 1.0 μg AFB1/mL
at pH 3.0 and 6.0 for a contact time of 60 min at room temperature. Analysis of
AFB1 in the samples was performed by high performance liquid
chromatography. AFB1 adsorption by the products ranged from 45.5% to
69.4% at pH 3.0 and from 24.0% to 63.8% at pH 6.0. The higher percentages (p
< 0.05) of AFB1 binding at both pH values were achieved with
products containing hydrolyzed yeast cells or yeast cell walls rather than
intact cells. The AFB1 binding percentages of BFR were 55.0 ± 5.0% at
pH 3.0 and 49.2 ± 4.5% at pH 6.0, which was not significantly different (p >
0.05) from commercial products containing inactive intact yeast cells. The
results of this trial indicate that the yeast-based products tested, especially
the BFR, have potential applications in animal feeds as a suitable biological
method for reducing the adverse effects of aflatoxins.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.