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Whole rice contains several fat‐soluble phytochemicals such as tocopherols, tocotrienols, and γ‐oryzanol which have been reported to possess beneficial health properties. This study was conducted to determine whether brown rice belonging to indica and japonica subspecies were distinguishable from each other regarding the concentration of these compounds by analyzing 32 genotypes. The fat‐soluble compounds were analyzed by normal‐phase HPLC in a single run. The variability of the compounds analyzed was high, but the mean content of γ‐oryzanol across all samples was significantly higher (P < 0.01) in japonica (246.3 mg/kg) than in indica rice (190.1 mg/kg). Similar differences were found for total vitamin E contents which were 24.2 mg/kg in japonica and 17.1 mg/kg in indica rice, respectively. In japonica rice, α‐tocopherol, α‐tocotrienol, and γ‐tocotrienol were the most abundant homologs, while in indica rice the most abundant were γ‐tocotrienol, α‐tocopherol, and α‐tocotrienol. A significant Pearson coefficient (0.80, P < 0.001) between α‐tocopherol and α‐tocotrienol levels was found, independent of the subspecies. Both compounds were positively correlated to total tocols and γ‐oryzanol contents. Although more studies are needed to evaluate the interference of growing rice in different environments and multiple years, the present study provided information on natural variations of the vitamin E isomers and the γ‐oryzanol contents in different rice genotypes.

Despite nutritional claims and other advantages that parboiled rice possesses, its consumption is still low in Brazil mainly because of the local preference for milled rice. In the first part of this study, the acceptability of five commercial brands of parboiled and milled rice was assessed by 100 consumers and similar acceptance levels were observed, evidencing that consumers might not reject parboiled rice for its sensory properties. In the second part, 286 consumers were surveyed about their eating habits and attitude towards rice, focusing especially the nutritional value, sensory and convenience aspects of parboiled rice. In the survey 35% of the respondents were unaware of parboiled rice. Cluster analysis performed on data grouped consumers in one segment with positive attitude and in two other segments with negative attitude towards parboiled rice. Segmentation was mainly because of negative beliefs about the sensory characteristics of the product, although its nutritional claims were unknown to most respondents, revealing the need of marketing efforts focused on informing consumers the health benefits and convenience of eating parboiled rice.

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In the present study, the cells of Bifidobacterium animalis subsp. lactis (BI-01) and Lactobacillus acidophilus (LAC-04) were encapsulated in cocoa butter using spray-chilling technology. Survival assays were conducted to evaluate the resistance of the probiotics to the spray-chilling process, their resistance to the simulated gastric and intestinal fluids (SGF and SIF), and their stability during 90 days of storage. The viability of the cells was not affected by microencapsulation. The free and encapsulated cells of B. animalis subsp. lactis were resistant to both SGF and SIF. The micro-encapsulated cells of L. acidophilus were more resistant to SGF and SIF than the free cells; the viability of the encapsulated cells was enhanced by 67%, while the free cells reached the detection limit of the method (103 CFU/g). The encapsulated probiotics were unstable when they were stored at 20 °C. The population of encapsulated L. acidophilus decreased drastically when they were stored at 7 °C; only 20% of cells were viable after 90 days of storage. The percentage of viable cells of the encapsulated B. animalis subsp.lactis, however, was 72% after the same period of storage. Promising results were obtained when the microparticles were stored at −18 °C; the freeze granted 90 days of shelf life to the encapsulated cells. These results suggest that the spray-chilling process using cocoa butter as carrier protects L. acidophilus from gastrointestinal fluids. However, the viability of the cells during storage must be improved.

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Probiotics are live micro-organisms that bring benefits to consumer health. However, to exert these benefits, the micro-organisms must remain viable during processing and for the shelf-life of food (with a recommended number of viable cells above 106 UFC/g) and must be resistant to gastrointestinal (GI) fluids. However, in general, probiotic cultures have problems related to their viability, stability and depreciation of the sensory acceptability of foods through the production of off-flavours. Overcoming these problems required considerable research effort. As a result, numerous studies have shown that microencapsulation is an alternative for probiotics to provide stability, viability, no proliferation in food and, additionally, controlled release in the intestine. There are still many challenges in this area, such as determining the mechanisms of release of the content of the microcapsules, development of low-cost enteric polymer microcapsules, development of symbiotic microcapsules and conferring thermal stability to probiotic cultures. In this context, this review aims at demonstrating the advances available in microencapsulation of probiotics.