Diarrhea cases in Indonesia were 270/1000 population or 7,265,013 cases occurred at all ages with a mortality rate (CFR) of 4.76% in 2018. Treatment of diarrhea can be done by giving antibiotics such as Cefotaxime, Ciprofloxacin, Gentamicin, Ampicillin, Metronidazole, Co-trioxide, Ceftriaxone, and Erythromycin. However, inappropriate use of antibiotics can lead to bacterial resistance. Turmeric is a medicinal plant that contains curcumin and essential oils that can inhibit the growth of bacteria. There are 24 essential oils in turmeric, which are dominated by turmerone (35.16%), ar-turmerone (25.20%), curlon (18.21%), and ar-curcumin (3.74%). Turmeric water obtained through juice has the potential as an antibacterial agent. This study is a pure experiment using a post-test-only control group design by examining the effect of turmeric juice (Curcuma domestica Val.) as an antibacterial of Escherichia coli bacteria. The results showed the ability of turmeric juice to inhibit the growth of Escherichia coli bacteria. The concentration of turmeric juice was able to produce an inhibition zone at concentrations ranging from 40% at 14 mm and the highest concentration at 100% at 24 mm. The results of the One Way Anova test showed that the results were not significant because the p-value>0. 05 (p-value=0.351). This means that the average diameter of the inhibition at the concentration of turmeric juice 40%, 55%, 70%, 85%, and 100% did not have a significant difference. Turmeric juice has effectiveness in inhibiting the growth of E.coli bacteria. Kata kunci:Antibakteri; Zona hambat; Air perasan kunyit. Kasus diare di Indonesia tahun 2019 sebanyak 270/1000 penduduk atau 7.265.013 kasus terjadi pada semua umur dengan angka kematian (CFR) sebanyak 4,76% pada tahun 2018. Pengobatan diare dapat dilakukan dengan pemberian antibiotic seperti Cefotaxime, Ciprofloxacin, Gentamisin, Ampicillin, Metronidazol, Kotrimokdazol, Seftriakson, dan Eritromisin. Akan tetapi penggunaan antibiotik tidak sesuai dosis dapat menyebabkan resistensi bakteri. Kunyit merupakan tanaman obat yang mengandung kurkumin dan minyak atsiri yang dapat menghambat pertumbuhan bakteri. Minyak esensial pada kunyit diketahui ada 24 macam yang didominasi oleh α-turmeron (35,16%), ar-turmeron (25,20%), kurlon (18,21%), dan ar-kurkumin (3,74%). Air kunyit yang diperoleh melalui perasan sangat berpotensi sebagai agen antibakteri. Penelitian ini merupakan eksperimen murni menggunakan rancangan post test only control group design dengan meneliti efek dari air perasan kunyit (Curcuma domestica Val.) sebagai antibakteri terhadap pertumbuhan bakteri Escherichia coli. Hasil penelitian menunjukkan kemampuan air perasan kunyit dalam menghambat pertumbuhan bakteri E.coli. Konsentrasi air perasan kunyit mampu memhasilkan zona hambat pada konsentrasi mulai 40% sebesar 14mm dan tertinggi konsentrasi 100 % sebesar 24 mm. Hasil uji One Way Anova menunjukkan hasil tidak signifikan karena p-value>0, 05 (p-value=0,351). Hal ini berarti rata-rata diameter daya hambat pada konsentrasi air ...
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