Every year, many dental restoration methods are carried out in the world and most of them do not succeed. High cost of these restorations and rejection possibility of the implants are main drawbacks. For this reason, a regenerative approach for repairing the damaged dentin-pulp complex or generating a new tissue is needed. In this study, the potential of threedimensional cellulose acetate/oxidized pullulan/gelatin-based dentin-like constructs containing 10 or 20% bioactive glass nanoparticles was studied to explore their potential for dentin regeneration. Three-dimensional nano biocomposite structures were prepared by freeze-drying/metal mold pressing methods and characterized by in vitro degradation analysis, water absorption capacity and porosity measurements, scanning electron microscopy, in vitro biomineralization analysis. During one-month incubation in phosphate buffered saline solution at 37 C, scaffolds lost about 25-30% of their weight and water absorption capacity gradually decreased with time. Scanning electron microscopy examinations showed that mean diameter of the tubular structures was about 420 mm and the distance between walls of the tubules was around 560 mm. Calcium phosphate precipitates were formed on scaffolds surfaces treated with simulated body fluid, which was enhanced by boron-modified bioactive glass addition. For cell culture studies human dental pulp stem cells were isolated from patient teeth. An improvement in cellular viability was observed for different groups over the incubation period with the highest human dental pulp stem cells viability on B7-20 scaffolds. ICP-OES analysis revealed that concentration of boron ion released from the scaffolds was between 0.2 and 1.1 mM, which was below toxic levels. Alkaline phosphatase activity and intracellular calcium amounts significantly increased 14 days after incubation with highest values in B14-10 group. Von Kossa staining revealed higher levels of mineral deposition in these groups. In this work, results indicated that developed dentin-like constructs are promising for dentin regeneration owing to presence of boron-modified bioactive glass nanoparticles.
Barrier membranes are used in periodontal tissue engineering for successful neo‐bone tissue formation and prevention of bacterial colonization. We aimed to prepare and characterize novel 7% boron‐modified bioactive glass (7B‐BG) containing bilayered membrane for this end. We hypothesized that presence of 7B‐BG could promote structural and biological properties of guided bone regeneration (GBR) membrane. Cellulose acetate (CA) layer was prepared by solvent casting, and functionally graded layer of CA/gelatin/BG nanoparticles was prepared by electrospinning. 0B‐BG, and 7B‐BG were synthesized by quick alkali‐mediated sol–gel method and were characterized by scanning electron microscopy (SEM) and Fourier‐transform Raman spectroscopy. Membranes were cross‐linked with glutaraldehyde to preserve their stability. SEM analysis showed the asymmetric nature of membranes consisting of a smooth membrane layer and a rough surface composed of 0B‐BG and 7B‐BG containing nanofibres. 7B‐BG addition increased surface wettability (from 110.5° ± 0.8 to 73.46° ± 7.6) and biodegradability of the membranes. Additionally, a significant increase in Ca–P layer formation was observed in 7B‐BG containing group after 1‐week incubation in stimulated body fluid. 7B‐BG incorporation resulted in a decrease in tensile strength and Young's modulus values. Human dental pulp stem cells showed better attachment, spreading, and proliferation on 7B‐BG containing bilayered membranes. Osteogenic differentiation analysis revealed higher alkaline phosphatase (ALP) enzyme activity of cells (~1.5‐fold), higher intracellular calcium deposition (approximately twofold), and higher calcium deposition revealed by Alizarin red staining on 7B‐BG containing bilayered membranes. Overall, results suggested that functionally graded bilayered membranes hold potential for GBR applications in regenerative dentistry.
In this study, we aimed to prepare and characterize porous scaffolds composed of pure and boron oxide (B2O3)-doped bioactive glass (BG) that were infiltrated by cellulose acetate-gelatin (CA-GE) polymer solution for bone tissue engineering applications. Composite scaffolds were cross-linked with glutaraldehyde after polymer coating to protect the structural integrity of the polymeric-coated scaffolds. The impact of B2O3 incorporation into BG-polymer porous scaffolds on the cross-sectional morphology, porosity, mechanical properties, degradation and bioactivity of the scaffolds was investigated. Human dental pulp stem cells (hDPSCs) were enzymatically isolated and used for cell culture studies. According to scanning electron microscope analysis, the porous structure of the scaffolds was preserved after polymer coating. After polymer infiltration, the porosity of the scaffolds decreased from 64.2% to 59.35% for pure BG scaffolds and from 67.3% to 58.9% for B2O3-doped scaffolds. Meanwhile, their compressive strengths increased from 0.13 to 0.57 MPa and from 0.20 to 0.82 MPa, respectively. After polymer infiltration, 7% B2O3-incorporated BG scaffolds had higher weight loss and Ca–P layer deposition than pure BG scaffolds, after 14 d of incubation in simulated body fluid at 37 °C. Higher attachment and proliferation of hDPSCs were observed on 7% B2O3-BG-CA/GE scaffolds. In addition, the alkaline phosphatase activity of the cells was about 1.25-fold higher in this group than that observed on BG-CA/GE scaffolds after 14 d of incubation in osteogenic medium, while their intracellular calcium amounts were 1.7-fold higher than observed on BG-CA/GE after 7 d of incubation in osteogenic medium. Our results suggested that porous cellulose acetate-gelatin-coated boron-BG scaffolds hold promise for bone tissue engineering applications.
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