Peach [Prunus persica (L.) Batsch] cDNA libraries have been constructed from RNA isolated from immature (30 days after bloom) and ripe fruit. cDNA clones of interest have been identified by differential hybridization among the cDNAs of various peach cultivars or from several stages in fruit development. In addition, several clones were isolated by low stringency hybridizations with oligonucleotides derived from a tomato polygalacturonase cDNA sequence and a cucumber peroxidase amino acid sequence. The pattern of accumulation of the corresponding mRNAs during fruit development was examined by RNA gel-blot analyses in the commercial cultivar Suncrest. Three cDNA clones, pch201, pch307, and pch313, were related to mRNAs that accumulate during the softening stage of fruit development. cDNA clones pchl03, pch205, and pch306 were related to an mRNAs that increase in abundance throughout development, with maximum levels in ripe fruit. cDNA clones pch104 and pch202 were related to mRNAs that exhibit maximum abundance in midfruit development, and clone pch108 was related to mRNA that decreases as the fruit matures. Southern analyses indicated that seven of the cDNAs are represented by only a few genes, while pch104 detects a repetitive family, and pch307 detects a small family of genes. These clones will provide the initial source of genes to manipulate and affect fruit development.
We are interested in identifying and isolating genes which affect the rate of softening in peach fruit. It may be possible through the engineering of these genes to delay or extend the softening. This could ultimately allow for the harvest and transport of more mature, higher quality fruit. The clone, pch313, was isolated from a ripe peach fruit cDNA library. RNA homologous to this clone is detected at a low abundance in fruit until softening when a >100 fold increase in abundance of the RNA occurs. Pch313 RNA is also detected 30 min after wounding leaf or fruit tissue and peaks in accumulation within 2-8 hours. Wound ethylene was measured from the same tissue and its rate of evolution paralleled the accumulation of the RNA. The cDNA was sequenced and found to have 78% sequence identity with pTom13, a tomato gene that is expressed during fruit ripening and wounding (Holdsworth et al., NAR 15:731-739, 1987). To determine the universality of pch313 related gene expression, RNA accumulation was measured in other fruits during softening, and in leaf tissue upon wounding.
We are interested in identifying and isolating genes which affect the rate of softening in peach fruit. It may be possible through the engineering of these genes to delay or extend the softening. This could ultimately allow for the harvest and transport of more mature, higher quality fruit. The clone, pch313, was isolated from a ripe peach fruit cDNA library. RNA homologous to this clone is detected at a low abundance in fruit until softening when a >100 fold increase in abundance of the RNA occurs. Pch313 RNA is also detected 30 min after wounding leaf or fruit tissue and peaks in accumulation within 2-8 hours. Wound ethylene was measured from the same tissue and its rate of evolution paralleled the accumulation of the RNA. The cDNA was sequenced and found to have 78% sequence identity with pTom13, a tomato gene that is expressed during fruit ripening and wounding (Holdsworth et al., NAR 15:731-739, 1987). To determine the universality of pch313 related gene expression, RNA accumulation was measured in other fruits during softening, and in leaf tissue upon wounding.
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