Comparison of High and Low Quality Peach Fruit Cultivars by Differential Hybridization of a Cdna Library

Callahan, Ann M.; Morgens, Peter H.; Scorza, R.; Mante, Seth; Cordts, John; Cohen, Reuben A.

doi:10.17660/actahortic.1989.254.7

Acta Hortic.

1989

DOI: 10.17660/actahortic.1989.254.7

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Comparison of High and Low Quality Peach Fruit Cultivars by Differential Hybridization of a Cdna Library

Ann M. Callahan¹,

Peter H. Morgens²,

R. Scorza³

et al.

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1993

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“…Here we report the cloning of some cDNAs whose corresponding mRNAs either accumulate or decrease during peach fruit development in the commercial quality cultivar Suncrest. Preliminary results have been presented previously (Callahan et al, 1989b(Callahan et al, , 1991.…”

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confidence: 95%

Isolation and Initial Characterization of cDNAs for mRNAs Regulated during Peach Fruit Development

Callahan¹,

Morgens²,

Cohen³

1993

jashs

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Peach [Prunus persica (L.) Batsch] cDNA libraries have been constructed from RNA isolated from immature (30 days after bloom) and ripe fruit. cDNA clones of interest have been identified by differential hybridization among the cDNAs of various peach cultivars or from several stages in fruit development. In addition, several clones were isolated by low stringency hybridizations with oligonucleotides derived from a tomato polygalacturonase cDNA sequence and a cucumber peroxidase amino acid sequence. The pattern of accumulation of the corresponding mRNAs during fruit development was examined by RNA gel-blot analyses in the commercial cultivar Suncrest. Three cDNA clones, pch201, pch307, and pch313, were related to mRNAs that accumulate during the softening stage of fruit development. cDNA clones pchl03, pch205, and pch306 were related to an mRNAs that increase in abundance throughout development, with maximum levels in ripe fruit. cDNA clones pch104 and pch202 were related to mRNAs that exhibit maximum abundance in midfruit development, and clone pch108 was related to mRNA that decreases as the fruit matures. Southern analyses indicated that seven of the cDNAs are represented by only a few genes, while pch104 detects a repetitive family, and pch307 detects a small family of genes. These clones will provide the initial source of genes to manipulate and affect fruit development.

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mentioning

confidence: 95%

Isolation and Initial Characterization of cDNAs for mRNAs Regulated during Peach Fruit Development

Callahan¹,

Morgens²,

Cohen³

1993

jashs

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show abstract

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Comparison of High and Low Quality Peach Fruit Cultivars by Differential Hybridization of a Cdna Library

Cited by 1 publication

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Isolation and Initial Characterization of cDNAs for mRNAs Regulated during Peach Fruit Development

Isolation and Initial Characterization of cDNAs for mRNAs Regulated during Peach Fruit Development

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