Significance and impact of the study: Microorganisms such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Enteritidis and S. enterica subsp. enterica serovar Typhimurium can cause contamination in fresh or processed food products. The use of natural compounds, for instance carvacrol, can be an alternative to the use of synthetic bactericides. Our data demonstrate that the carvacrol nanoemulsion (NECV) had stability for up to 90 days, promoted inhibitory activity against all evaluated microorganisms , and improved antimicrobial activity against P. aeruginosa, S. aureus and S. Typhimurium when compared to free oil. These findings suggest that NECV can be evaluated as a food preservative to promote antimicrobial activity.
The objective of the study was to determine the antimicrobial activity antibiofilm and to identify the main components of the essential oil (EO) obtained from the leaves of Cymbopogon flexuosus. The antibacterial and antibiofilm activity was determined against Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Salmonella Typhimurium ATCC 14028 and Listeria monocytogenes ATCC 19117. The effect of EO on biofilm was evaluated by quantifying viable cell number (CFU) and biomass by crystal violet (CV) analysis. The composition of the essential oils was determined by GC / FID and GC / MS. The results showed action against L. monocytogenes, S. aureus and S. Typhimurium with MIC and MBC values of 3.9 µL mL-1 , thus showing satisfactory antimicrobial activity, given this was the lowest concentration tested. For the antibiofilm activity, a significant reduction (P < 0.05) was observed for S. typhimurium and S. aureus. Biofilm biomass significantly reduced only for S. aureus and P. aeruginosa. EO presented the geranial and neral isomers as major components.
O objetivo deste estudo foi determinar a atividade antimicrobiana e antioxidante do óleo essencial (OE) obtido das folhas de Cymbopogon flexuosus (capimlimão da Índia Oriental). A atividade antimicrobiana foi desenvolvida contra Pseudomonas aeruginosa ATCC 27853 e Listeria monocytogenes ATCC 19117 a fim de iniciar estudos para o desenvolvimento de um detergente-sanificante. A eficiência do OE foi detectada pelo método da Concentração Mínima Inibitória (CMI) e Concentração Mínima Bactericida (CMB). Todos os ensaios foram realizados em triplicata e em três repetições. A atividade antioxidante do OE foi avaliada pelo consumo do radical livre DPPH comparado com os antioxidantes sintéticos BHT e ácido gálico. Foram testadas oito concentrações do OE, partindo de 3,9 à 1000µL mL-1. Os resultados apontaram ação contra L. monocytogenes, com valores de CMI e CMB de 3,9μL mL-1 apresentando atividade antimicrobiana satisfatória, devido esta ser a menor concentração testada. Nenhuma das concentrações testadas foi eficiente para inibir P. aeruginosa. Avaliando o combate a radicais livres verificou-se excelente eficiência em todas as concentrações do OE analisadas, demostrando grande potencial antioxidante. Esses resultados refletem eficiente atividade antioxidante e antimicrobiana do OE de Cymbopogon flexuosus contra L. monocytogenes indicando o possível uso na formulação de alimentos e/ou solução detergente-sanificante.
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