Background. To reduce morbidity to cleft patients, new approaches have been developed and here, we report for the first time the use of deciduous dental pulp stem cells (DDPSC) associated with a hydroxyapatite-collagen sponge (Bio-Oss Collagen® 250 mg, Geistlich) for closing alveolar defects during secondary dental eruption, further comparing these results to historical controls. Methods. Six patients, aged 8 to 12, were selected. Autologous DDPSC were isolated from each patient, then associated with the biomaterial and this bone tissue engineered set was used to fill the alveolar defect. Computed tomography was performed to assess both preoperative and 6- and 12-month postoperative outcomes. Overall morbidity was recorded. Historical controls consisted of sixteen patients previously selected and randomly assigned to group one (rhBMP-2) or group two (iliac crest bone graft). Results. DDPSC could be isolated and characterized as mesenchymal stem cells. Progressive alveolar bone union has occurred in all patients. Similarly to group two 75.4%, SD±4.0, p>0.999, but statistically different from group one (59.6%, SD±9.9, p=0.001), completion of the defect with 75.6% (SD±4.8) of bone filling was detected 6 months postoperatively. Dental eruption routinely occurred in 66.7% of patients. No complications were detected, in comparison to significant swelling in 37.5% of group one patients and significant donor site pain in 87.5% of group two. Conclusion. For this selected group of patients, DDPSC therapy resulted in satisfactory bone healing with excellent feasibility and safety, which adds significantly to the prospect of stem cell use in clinical settings. Clinical Question/Level of Evidence. Therapeutic, II. This trial is registered with https://clinicaltrials.gov/ct2/show/NCT01932164?term=NCT01932164&rank=1.
Ezrin protein acts in the regulation of cytoskeletal and directly influences survival and tumor progression; there is an increase in its expression in metastatic cells and tissues in several types of cancer including colorectal cancer. 250 Patients with colorectal cancer submitted to surgery from 1995 to 2002. Protein expression was carried through by Tissue Micro Array immunohistochemical tests of paraffined neoplasic tissues and associated with clinical variables. Differentiation degree, lymph node invasion, metastasis at diagnosis, and palliative surgery were associated to a higher expression of the protein and survival. Higher expression of the Ezrin correlates with tumor aggressiveness and worse prognosis for colorectal cancer.
Background Autologous fat grafting (AFG) has been used in surgical practice as a filling method. However, controversies remain on the specifics of this technique. So far, few relevant experimental large animal studies have objectively assessed factors related to AFG integration. Objectives This study uses an experimental medium-sized animal model to compare the feasibility of AFG collected using two different techniques with instruments of distinct thicknesses. Methods Twenty minipigs (Sus scropha domesticus) were subjected to AFG harvesting via en bloc resection using 3 (Group I) and 5 mm diameter (Group II) round punch blades (PB) and liposuction (LS) with 3 (Group III) and 5 mm diameter cannulas (Group IV). Both samples were grafted intramuscularly (biceps femoralis). Hematoxylin and eosin (HE) staining was used to identify intact adipocytes, fat necrosis, fibrosis, inflammation, and oil cysts. Immunohistochemical staining (IHC) (perilipin-A, TNF-α, CD31) were utilized to quantify the feasibility of adipocytes, tissue necrosis, neoangiogenesis, respectively. Results HE analysis showed that fat necrosis/histiocyte presence were significant lower in the AFG harvested using a PB than in LS. For perilipin-A, a statistical difference was observed between subgroups I and III (p=0.001) and I and IV (p=0.004). Instrument diameter was seen to have no effect on graft integration, as seen in comparisons between groups II and III (p=0.059) and II and IV (p=0.132). Conclusion In this experimental study, fat collected using a PB demonstrated higher adipocyte viability than fat collected with LS. The diameter of the collection instruments, whether PB or LS, had no effect on graft integration.
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