This study aimed to detect Toxoplasma gondii in the milk of dairy sheep in the Western mesorregion of state of Santa Catarina by bioassay (22 milk samples from eight ewes seropositive; IFA ≥256) and PCR [for the detection of agent in the brains of mice inoculated on bioassay and directly from milk (108 samples from 42 seropositive ewes (IFA, ≥64) in different lactation periods)]. T. gondii DNA was detected in mice brains inoculated with milk from eight sheep (a sample of the 45th day of lactation and seven in the collection of 90th day) and directly from the milk in samples of the second collection (90 days) in five animals. Taking into account both assays, from a total of 42 ewes in lactation and seropositive for T. gondii, 30.95% (13/42) of the animals presented evidences of T. gondii presence in milk. Positive PCR samples were sequenced and the results confirmed ≥97% identity with the membrane antigen P22 gene of T. gondii. The results showed that T. gondii is present in the milk of sheep, representing a possible source of infection to humans through the consumption of milk "in natura" and/or derivatives, besides the possibility of lactogenic transmission to lambs.
RESUMO:A carne ovina possui grande potencial de transmissão do Toxoplasma gondii para os seres humanos e demais animais carnívoros, principalmente quando consumida crua ou mal passada. Com o objetivo de avaliar o efeito do resfriamento e congelamento sobre a viabilidade de cistos deste parasito em tecido muscular ovino, foram utilizados cortes cárneos de um cordeiro naturalmente infectado por T. gondii, com titulação de 1:256 na RIFI. Coração, fígado, diafragma e cérebro foram coletados em formol 10% para histopatológico. Coração e cortes musculares da paleta, costela e pernil foram usados nos tratamentos "in natura", resfriados a 7°C por 24h e congelados a -10°C por 12h, 60h e 120h. Após cada tratamento, foram coletados 50g dos cortes e 16g do coração para realização do bioensaio em camundongos e PCR. Após oito semanas, os camundongos foram eutanasiados e coletados órgãos para histopatológico, cérebro para "squash" e PCR e soro para RIFI. Foram positivos dois camundongos inoculados com amostras "in natura" e um inoculado com amostra após o resfriamento. Não foi possível verificar o efeito do congelamento sobe a viabilidade do T. gondii. Palavras-chave: carne; cisto; ovino; Toxoplasma gondii; tratamento térmico ABSTRACT: The lamb has great Toxoplasma gondii potential transmission to humans and other carnivorous animals, especially when consumed raw or undercooked. In order to evaluate the effect of cooling and freezing on the viability of cysts of this parasite in sheep muscle tissue, were used meat cuts of lamb naturally infected with T. gondii, with titer of 1: 256 at IFA. Heart, liver, diaphragm and brain were collected in 10% formalin for histopathological. Heart and muscle cuts from the palette, rib and ham were used in treatments "in natura", cooled to 7°C for 24 hours and frozen at -10°C for 12h, 60h and 120h. After each treatment were collected 50g of heart cuts and 16g for bioassay in mice and PCR. After eight weeks, the mice were euthanized and organs collected for histopathologic brain to "squash" and PCR and serum to IFA. Positive were two mice inoculated with samples "in natura" and an inoculated with sample after cooling. Could not verify the effect of freezing up the viability of T. gondii.
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