Periodontal diseases are the most common diseases in veterinary medicine. The first clinical finding is chewing difficulty, saliva flow and bad oral odor. It further develops into plaque and tartar formation, gingival inflammation and hemorrhagic appearance of the gingiva, periodontal pockets formation, alveolar bone resorption and tooth loss. In this study an evaluation has been made to determine which degree reflects on the parameters of systemic inflammatory reaction with special attention to IL-6 (Interleukine-6), CRP (C-reactive protein), osteopontin, superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GPx) and Ig (Immunglobulins = Total protein – Albumin) and hematological parameters in dogs with periodontitis. Two groups have been defined in this study. The first group included 10 healthy and owned dogs as a control group. The second group consisted of 10 owned dogs with moderate-severe periodontitis. The difference between monocyte (p <0.001) and neutrophil (p <0.05) counts was found to be significant. In addition, the difference between SOD, MDA, glutathione peroxidase, CRP, IL-6 measurements in group 1 and group 2 was significant. (p <0.001). The level of osteopontin in moderate-severe periodontitis cases was found significantly higher than the level measured in the healthy group. Measured values in the moderate-severe periodontitis cases are higher than the healthy group in terms of CRP, IL-6, and osteopontin levels. Increasing severity of periodontitis was associated with changes in oxidative stress parameters: increased MDA, decreased SOD and glutathione peroxidase levels. These differences provide important information about the evaluation of the cellular responses. There is a need for continued research into the systemic impact of periodontal disease.
Tocopherols, the major forms of vitamin E, are a family of fat-soluble phenolic compounds. Each tocopherol contains a chromanol ring system and a phytyl chain containing 16 carbons. Tocopherols, as effective antioxidants, have been proposed to protect against carcinogenesis. Colon carsinoma cell line were treated with tocopherol-α (0, 3.12, 6.25, 12.5, 25, 50, 100, 200 uM). Cell viability and migration were examined. Cell viability was determined MTT assay and cell migration was determined by wound healing assay. Caco-2 cells were treated with for 24 h, 48 h and 72 h incubation. There is a significant decrease was observed at 50, 100 and 200 µM for 48 h and 72 h incubation on cell viability in the MTT assay. Wound healing method observed decrease on migration at 12, 5, 25 and 50 µM in 24 h. These results suggest that tocopherol-α have promising antiproliferative effect on cell viability for research on cancer.
Alpha tocopherol is the most common and biologically active form of Vitamin E. The aim of this study was to evaulate the possible antiproliferative effect of alpha tocopherol on F-98 Glioblastoma cells. F-98 glioblastoma cell line was seeded at a density of 50.000/mL per well in 96 well plates in 100 µL medium DMEM. Cells treated with Alpha tocopherol (200,100, 50, 25, 12.5, 6.2, 3.3 µM) for 24 h, 48 h and 72 h incubation. Measurement of Alpha tocopherol treated and control groups' cell proliferation performed with MTT assay and Wound Healing assay was employed to show migration capacity. MTT Assay data shown are there was significant change in cell viability in 24 h, 48 h and 72 h. However significant decrease was observed at 50, 100 and 200 µM. In the present study, antiproliferative effect of alpha tocopherol was observed via wound healing assay. Our results here show that Alpha Tocopherol maybe a possible avenue for brain cancer treatment.
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