Mycoplasmas are an important class of bacteria associated with caprine pneumonia. Some of the main species involved are M. mycoides subsp. capri, M. capricolum subsp. capricolum, and M. capricolum subsp. capripneumoniae. In addition, there are frequent reports of M. arginini involvement. In the present study an attempt was made to see the association of various mollicutes in caprine pneumonia. In total 244 pneumonic goat lung samples collected over a 10-year period (2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010)(2011)(2012) were screened for the isolation and identification of associated mollicutes. The identification was done on the basis of biochemical tests, growth inhibition test, PCR, and sequencing. The most frequent isolates were Acholeplasma (20/244, i.e. 8.19%) and Mycoplasma arginini (13/244, i.e. 5.3%), followed by M. mycoides subsp. capri (1/244) and M. agalactiae (1/244). Seven representative isolates of M. arginini were subjected to partial sequencing of the rpoB gene. On phylogenic analysis, M. arginini isolates were found to be highly identical, exhibiting >98% identity with that of the standard M. arginini (ATCC 23243) strain. The results encourage exploration of the role of M. arginini in goat pneumonia in much detail, due to its high frequency involvement.
| Mycoplasma mycoides subspecies capri (Mmc), the causative agent of caprine pleuro pneumonia/contagious agalactia is a major pathogen affecting goats worldwide. Development of specific immunodiagnostic assays for Mmc is often hampered by interspecies cross-reactivity with other caprine mycoplasmas and the intra species antigenic variability. The study presents a comparative and subtractive proteomic analysis to identify specific, conserved and immunogenic lipoproteins from Mmc proteome. Analysis of 896 proteins of Mmc strain 95010 predicted 72 lipoproteins by lipoP1.0 server. BLAST analysis revealed 17 putative non cross-reactive lipoproteins out of which seven were found to be conserved within the species. Further computational workflow employing ExPASy protein analysis tools and B cell epitope prediction softwares identified five lipoproteins suitable for development of immunodiagnostics.
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