Mycoplasmas are an important class of bacteria associated with caprine pneumonia. Some of the main species involved are M. mycoides subsp. capri, M. capricolum subsp. capricolum, and M. capricolum subsp. capripneumoniae. In addition, there are frequent reports of M. arginini involvement. In the present study an attempt was made to see the association of various mollicutes in caprine pneumonia. In total 244 pneumonic goat lung samples collected over a 10-year period (2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010)(2011)(2012) were screened for the isolation and identification of associated mollicutes. The identification was done on the basis of biochemical tests, growth inhibition test, PCR, and sequencing. The most frequent isolates were Acholeplasma (20/244, i.e. 8.19%) and Mycoplasma arginini (13/244, i.e. 5.3%), followed by M. mycoides subsp. capri (1/244) and M. agalactiae (1/244). Seven representative isolates of M. arginini were subjected to partial sequencing of the rpoB gene. On phylogenic analysis, M. arginini isolates were found to be highly identical, exhibiting >98% identity with that of the standard M. arginini (ATCC 23243) strain. The results encourage exploration of the role of M. arginini in goat pneumonia in much detail, due to its high frequency involvement.
Aim:The present study was undertaken to clone, express and study the immunogenicity of P67 protein of Mycoplasma leachii.Materials and Methods:P67 gene was amplified from genomic DNA of M. leachii. The polymerase chain reaction (PCR) product was inserted in pRham N-His SUMO Kan vector and was used to transform competent Escherichia cloni 10G cells. Recombinant protein expression was done by inducing cells with 0.2% Rhamnose. Purification was done using nickel nitrilotriacetic acid affinity chromatography. Western blot and dot blot analysis were performed to assess the immunoreactivity of P67 protein.Results:PCR amplicon size of P67 gene was found to be 1500 base pair. The size of the fusion protein with SUMO tag was 79 kDa in sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis. The recombinant P67 fusion protein expressed in pRham N-His SUMO Kan vector was found to be immunogenic in both western blot and dot blot analysis.Conclusion:Western blot and dot blot analysis of P67 protein of M. leachii revealed that the protein is immunogenic. Further work is needed to evaluate the role of P67 antigen of M. leachii as an immunodiagnostic agent.
| Mycoplasma bovis is an important bovine pathogen associated with calf pneumonia, mastitis, arthritis and sepicemia. It is known to persist in phagocytic and nonphagocytic cells and disseminate to various organs causing systemic infection. In this study, the effect of live M. bovis on pro-inflammatory cytokine production by bovine peripheral blood mononuclear cells was assessed by relative quantification. Temporal change in cytokine levels was assessed at 8 hourly intervals. IL-6 was upregulated (9.32 fold at 24h) by live M. bovis at MOI of 1: 10, while TNF-α levels remained unchanged over time. IL-1 and TNF-α levels were not altered by M. bovis as compared to the control group. This showed that pro-inflammatory cytokines are not much upregulated at low multiplicity of infection.
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