Three hundred eight isolates of Magnaporthe grisea were collected from rice plants in 14 counties of Yunnan Province, China in 1988-89. Each isolate was tested for mating type and fertility with Matl-1 and Matt-2 female fertile tester strains. Three percent of these isolates consisted of Matt-1, and 23% of Matt-2. The remaining 74% isolates did not produce perithecia in crosses with both Matl-1 and Matl-2 fertile testers. The percentage of fertile isolates in each county ranged from 90% in Lincang to 0% in Lijiang, Dali, and Honghe. Almost all the isolates were differentiated into races based on the reaction of Japanese differential cultivars. Sixteen races were identified within the fertile isolates. Isolates of both mating types were found in two counties, Qujing and Xishuangbanna, and those in Xishuangbanna were slightly interfertile. In contrast, crosses between the hermaphroditic isolate CHNOS37-1-1 from Xishuangbanna and three male isolates from other counties led to the successful production of large numbers of perithecia containing viable ascospores. The crosses were fertile enough to permit the isolation of germinating ascospores. The southwestern area where fertile blast fungi were isolated with a considerable frequency, coincided with the area with a high diversity of rice cultivars.
Ribosomes were isolated from four snow mold fungi (Pythium paddicum, P. iwayamai, Typhula incarnata and Fusarium nivale) and wheat seedlings, and their proteins were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). No difference was found in electrophoretic patterns between P. paddicum and P. iwayamai. However, there were several differences among two Pythium species, T. incarnata, F. nivale and wheat. The ribosomes were isolated also from wheat seedlings infected with P. paddicum, T. incarnata and F. nivale individually, and their proteins were analyzed by SDS-PAGE. The distinctive fungal ribosomal protein bands were detected in all patterns obtained from the infected wheat plants. The possibility of the ribosomal protein-based assay to detect snow mold fungi in wheat plants is suggested.
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