A dielectric barrier discharge generated by flowing inert gas (helium) ionized by a high-voltage source through a cylindrical reactor working at atmospheric pressure has been studied and an electrical model characterizing this discharge is proposed. A sinusoidal voltage of up to 2 kV peak to peak with frequencies from 10 to 125 kHz has been applied to the discharge electrodes. The proposed model considers the geometry of the reactor and dielectric materials. From experimental and analytical results, a semi-empirical relation of the breakdown voltage is presented as a function of the operating frequency. The microdischarge regime is characterized by a dynamic equivalent capacitance.

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The aim of the present study is to determine the deoxyribonucleic acid (DNA) damage by cells exposed to atmospheric pressure non-thermal plasma (APNTP). Mouse leukocytes embedded in agarose were exposed to the plasma at two different distances from a helium plasma needle outlet and during three different exposure periods. Damage was assessed by the single cell gel electrophoresis assay. The results indicate that, at 0.1 cm from the plasma needle, the exposure caused complete DNA fragmentation determined by the presence of so called "clouds". Samples exposed at 0.5 cm from the slide sample surface presented damage proportional to the exposure periods in terms of tail intensity, tail moment and "clouds" frequency. Studies performed with alkaline single cell gel electrophoresis assay to determine DNA breaks and alkali-labile sites, indicates that DNA damage produced by exposure to APNTP was caused mainly by oxidative radicals, rather than by UV light which causes cyclobutane pyrimidine dimers. These results allow us to conclude that plasma needle induced DNA breaks in mice leukocytes proportionally to exposure time.

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