Background: During past decades, liposomes have emerged as efficient carriers for drugs, diagnostics, vaccines, nutrients and other bioactive agents. Liposomes, the spherical vesicles consisting of phospholipids bilayer have the ability to encapsulate both lipophilic and hydrophilic drugs. Extensive studies have been done in the past for investigating a number of drugs and genes for controlled release with liposomal formulation. Liposomes have also been investigated for their use in cancer treatment. Liposomes offer various advantages because of their biocompatible, biodegradable, nontoxic and non-immunogenic nature. Methods: Liposomes have cell-specific targeting with important applications in the fields of nanotechnology like cancer therapy, diagnosis, gene delivery, cosmetics, agriculture and in food technology. They are prepared by various methods like sonication method, ethanol injection method, lipid film hydration method, micro-emulsion method. Conclusion: This review will provide an overview of classification, the various formulation methods, characterization, patented formulations and applications of liposomes with future prospects.
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