Phospholipid vesicles are commonly used as biomimetics in the investigation of the interaction of various species with cell membranes. In this paper we present a 31 P NMR investigation of a simple vesicle system using a paramagnetic shift reagent to probe the inner and outer layers of the lipid bilayer. Time-dependent changes in the 31 P NMR signal are observed, which differ whether the paramagnetic species is inside or outside the vesicle, and on the choice of buffer solution used. An interpretation of these results is given in terms of the interaction of the paramagnetic shift reagent with the lipids.3
Size-exclusion chromatographic NMR
is the application of size-exclusion
chromatographic media to modify the observed diffusion properties
of analyte molecules, as measured by diffusion ordered spectroscopy.
This Article demonstrates the application of this method to isotropic
solutions of sunset yellow, which are known to be formed of large,
noncovalent aggregates. The results show a partitioning effect of
the aggregates between in-pore and free solution environments, which
is explained by size-exclusion behavior. The influence of pore size
on this partition is also noted.
The use of chromatographic stationary phases or solvent modifiers to modulate diffusion properties in NMR experiments is now well established. Their use can be to improve resolution in the diffusion domain or to provide an insight into analytemodifier interactions and hence the chromatography process. Here we extend previous work using size-exclusion chromatographic (SEC) stationary phases to the investigation of polymer mixtures. We demonstrate that similar diffusion modulation behaviour is observed with an SEC stationary phase which can be understood in terms of size-exclusion behaviour.3
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