Rice Bacterial Leaf Blight (BLB) is considered the most imperative disease among various dangerous maladies of rice in Pakistan. There is no any reliable source of resistance against this disease. Moreover, pathogen has a vast diversity in its population. So, first and most important step for its control is pathogen identification and characterization. Therefore, present studies were carried out for surveillance of disease and to collect disease specimens from Hyderabad and Tando-Muhammad Khan (TMK) Districts. Associated pathogen was isolated from collected samples by direct plating method. Fourteen cultures were purified by streaking method and were characterized on the basis of colony morphology, cell morphology and gram staining. The colonies were found to be large, medium and small. Their shapes were filamentous, irregular and circular. They were found to be raised, undulate, entire and convex showing pale yellow, yellow, off white, reddish and creamy color and surface of most of the colonies was observed to be smooth.
The role of Alternaria sp on seed vigor of rapeseed, Brassica napus, was investigated. Seed samples were collected from rapeseed growing regions of Sindh province. The samples were processed for fungal recovery using blotter paper and agar plate methods. Both methods produced a number of parasitic and saprophytic fungi. Among the recovered fungi, Alternaria sp was predominant with 16% average infection. Similarly, effect of seed treatment with Topsin M. was examined in this experiment. It improved seed health significantly over inoculated seeds. The treated seeds showed greater germination (16.15%) with 83.69% more healthy seedling in comparison with inoculated seeds. Root and shoot systems were significantly improved in seedlings from treated seed compared to those from inoculated seeds. The results of this study suggest grading seed samples to reduce seed impurities and seed treatment (particularly with Topsin M) to eliminate seed borne pathogen, especially Alternaria from rapeseed to enhance production by the growers.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.