Describes the procedures involved in separating proteins or nucleic acids through disc electrophoresis.

2,6-Dibromophenol has been isolated from a luminous marine enteropneust, Balanoglossus biminiensis, found on intertidal beach areas at Sapelo Island, Georgia. This compound, responsible for the characteristic "iodoform-like" odor of these animals, is present in relatively large amounts; the estimated quantity per organism is 10 to 15 milligrams. Identity of the isolated substance as 2,6 dibromophenol is based on analyses of ultraviolet, infrared, and nuclear magnetic resonance spectra, mass spectrometry analysis, and on melting-point data.

A simple method has been devised for the extraction and purification of human growth hormone (HGH) from acetone-dried pituitary glands. The HGH, suitable for clinical use, is recovered in one fraction in a yield of 70-80 IU/g of pituitary powder. A step is included to preserve the glycoprotein hormones in the glands. The method can also be applied to extracting clinically useful HGH from embalmed pituitary glands in a yield of 13-22 IU/g of pituitary powder. For chemical, immunochemical or special clinical uses, HGH may be purified further by chromatography on DEAE-cellulose. (J Clin Endocr 29: 1456, 1969 M UCH OF THE human growth hormone (HGH) used clinically has been prepared by the method of Wilhelmi (1, 2) or of Raben (3). We have considered it desirable to modify the existing procedures so that the extraction and purification scheme is relatively simple and, at the same time, avoids exposing the hormone to conditions which tend to denature it. The procedure which we have developed meets these criteria and gives a good yield of HGH which is clinically useful and which may be purified further for chemical use and immunochemical studies.The method is based partly on a procedure described by Elrick et al. (4), and includes a step for the conservation of other hormonal activities in the pituitary gland. It is also adaptable to the extraction of HGH from pituitary glands taken from embalmed bodies. Materials and MethodsAH operations are carried out at 0-4 C unless otherwise noted.The human pituitary glands are stored in

A survey of the literature is presented on liquid chromatographic (LC) determination of tetracyclines in plasma, urine, and tissues of foodproducing animals. Food Safety and Inspection Service (FSIS) research on tetracyclines during 1973-1984 is discussed, including the thin layer chromatographic qualitative identification method for tissues. Research on development of LC column packings for trace level chromatography of tetracyclines and anhydrotetracyclines is also discussed. Data are shown for recovery, ruggedness testing, and analyst qualification studies on the tentative version of the LC method