Liquid Chromatographic Assay of Tetracyclines in Tissues of Food-Producing Animals

Abstract

A survey of the literature is presented on liquid chromatographic (LC) determination of tetracyclines in plasma, urine, and tissues of foodproducing animals. Food Safety and Inspection Service (FSIS) research on tetracyclines during 1973-1984 is discussed, including the thin layer chromatographic qualitative identification method for tissues. Research on development of LC column packings for trace level chromatography of tetracyclines and anhydrotetracyclines is also discussed. Data are shown for recovery, ruggedness testing, and analyst qualification studies on the tentative version of the LC method

Determination of tetracycline and its major degradation products by liquid chromatography with fluorescence detection

“…Tetracycline used in feed additives are even less pure. The source of the tetracycline used in feeds is the residual tetracycline found in the dried, ground mycelial powder harvested from fermentation tanks [1].…”

Trace analysis of oxytetracycline and tetracycline in milk by high-performance liquid chromatography

“…However, baseline drift, poor resolution, and severe peak tailing were consistently noted. Since it has been assumed (Knox and Jurand, 1975; Ashworth, 1985) that these effects are due to the presence of residual free silanol groups on the support surface, and/ or to metal ion contamination in the base silica of the bonded phases, attempts were made to develop another solvent system for TC elution that would be capable of blocking the reactive sites of the stationary phase, eliminating, thus, peak tailing and improving resolution.…”

“…Blocking agents added in mobile phase such as ethylenediaminetetraacetic acid (Mack and Ashworth, 1978;Ashworth 1985;Sporns et al, 1986;Chappell et al, 1986; Martinez and Shimoda, 1988) and/or alkyl-substituted ammonium compounds (Eksborg and Ekqvist, 1981; Moats, 1986;Khan et al, 1987) have been successfully used to reduce peak tailing. However, some authors have reported that such an addition was not needed (Eksborg et al, 1979;Hermansson, 1982;Onji et al, 1984;Botsoglou et al, 1984), whereas others have stated that new HPLC columns should be conditioned, before use, with chelating agents (Leenheer and Nelis, 1977; Sharma and Bevill, 1978).…”

“…Considerable progress has been made recently on TC analysis by the use of high-performance liquid chromatography. Various HPLC methods allowing sensitive, accurate, and precise quantitation of TCs in tissues (Sharma and Bevill, 1978; Onji et al, 1984;Ashworth, 1985; ! Laboratory of Milk and Milk Products.…”

Análise de resíduos dos antibióticos oxitetraciclina, tetraciclina, clortetraciclina e doxiciclina, em leite, por cromatografia líquida de alta eficiência

“…Um ensaio microbiológico reflete a concentração total, ou seja, não considera individualmente as tetraciclinas. Portanto, é necessário que se utilize um método de análise cromatográfico específico para as tetraciclinas (OKA et ai, 2000, TSAI & KONDO, 1994, OKA et ai , 1991, ASHWORTH, 1985. SHAND, 1998, OKA et ai , 2000.…”