Objective: to analyze the relationship between occupational stress and sleep quality in health professors. Methods: this is a cross-sectional study using a sociodemographic, occupational and Occupational Stress Scale and Pittsburg Sleep Quality Index. Results: male professors (57.9%), married (47.4%), and children (52.6%), living with the family (89.5%) and with a mean age of 34.7 years old predominated. In the sample, 63.2% had low-stress level and 57.9% had poor sleep quality, which was more affected by daytime sleepiness, sleep disturbances, and sleep duration. There was a significant and positive correlation between general stress level and use of sleeping medication. Conclusion: there was low level of stress and low quality sleep. Daytime sleepiness, sleep disturbances, and sleep duration are the factors that contribute most to the poor quality of sleep of health professors.