This research is aimed to quantitate and characterize the subtypes of tumour infiltrating lymphocytes (TILs), in particular the presence of FoxP3+ Tregs in different grades of oral squamous cell carcinoma using monospecific antibody staining of formalin-fixed, paraffin-embedded tissue sections. The correlation between tumour grade and the intensity of tumour infiltrating lymphocytes was tried to be tested, to assume a putative linkage between them. Thirty-four cases of histologically proven primary oral squamous cell carcinoma (OSCC) of different grades of differentiation were assorted to groups 1-3. Three-micron sections of tissue were cut and captured on electrically charged slides (Vision BioSystem, Mount Waverley, Australia) and stained using monospecific antibody against FoxP3+ Treg phenotype (dilution 1:40, Mouse monoclone No: 236A/E7, Ab 20034, IgG1; Abcam, Cambridge, UK). Automated protocols were employed for staining and scoring of staining intensity using Bond™ system (Vision BioSystem). Significant difference in staining intensities (Tregs) was noted among the histologic grades of tumour, where well-differentiated OSCC had significantly low expression of FoxP3+ Tregs in comparison with moderately and poorly differentiated tumours. A significant linear correlation was established between tumour grade and the intensity of TILs, where high grade tumours (poor differentiation) were more markedly infiltrated. There was also a significant positive correlation between FoxP3+ Tregs and TILs in cases studied. The correlation of these three variables noted in the study (FoxP3+ Tregs, tumour grade and TILs) and their significance in a meta-analysis may prove useful in targeting patients with high-risk neoplasms for more aggressive treatment protocols and management strategies.
J Oral Pathol Med (2013)42: 186–193
Background: The altered expression of syndecan‐1 (SD‐1), a transmembrane heparan sulfate proteoglycan, in ameloblastomas and cysts of odontogenic origin suggests that this molecule could have prognostic value in assessing the clinical outcome of those lesions. The purpose of this study was to analyze SD‐1 expression profile immunohistochemically in archival, paraffin‐embedded tissue sections of ameloblastomas and in common odontogenic cysts arising from the same locale.
Methods: SD‐1 expression was investigated in 32 ameloblastomas, 26 keratocystic odontogenic tumors (KCOT), and 21 dentigerous cysts from the archives of the histopathology laboratory which were routinely processed. The cases were reviewed and assessed according to the established criteria. Sections were immunostained with monoclonal antibody against SD‐1 (CD138). Sections of normal oral mucosa, site matched, were stained in parallel as positive controls. The plasma cells in sections served as internal positive controls.
Results: SD‐1 expression was observed in the epithelial and stromal elements of the sections, and the expression was significantly associated with the lesion’s extension and involvement of adjacent structures (P = 0.025). Stellate‐reticulum cells showed higher expression than the ameloblasts, which was at a significant level (P < 0.0001). Highly significant difference was reported among the three groups of lesion for the epithelial staining (P < 0.0001). The mean rank scores (Kruskal–Wallis test) of ameloblastomas were significantly lower than those of KCOT and dentigerous cysts. Non‐significant comparison was made between KCOT and dentigerous cyst groups.
Conclusions: The present study revealed SD‐1 immunoreactivity in the stromal cells of ameloblastoma, KCOT, and dentigerous cysts rather uniformly. This reported SD‐1 expression by the tumor stroma is considered to be associated with poor prognosis of the lesions.
An aggressive and fatal case of osteosarcoma of the mandible in a 19-year-old female is reported. Six weeks after the clinical appearance of the swelling, the patient died. This paper is unique in that the age of occurrence and the biologic behavior of the tumor were not consistent with the reported literature. The case report is followed by a brief review of osteosarcoma of the jaw with a note on its clinical presentation, diverse radiologic appearance, varied histopathologic picture, and prognosis.
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