The present study focuses on an efficient eco-friendly method for reducing graphene oxide (rGO) using Coffea arabica leaf extract for bio-medical applications for the first time to the best of our knowledge. The reduction of graphene oxide (GO) using Coffea arabica leaves was verified through Raman, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM). The XRD peaks corresponding to GO at 2 =10º have dissapeared on reduction of GO to rGO and the formation of rGO was verified through a new broad peak at 2 =26º. FTIR revealed functional group changes in reducing GO to rGO. The SEM images of rGO showed a ribbed form instead of the rigid appearance of the GO flakes. The analysis revealed that the current green method is a feasible method for reducing GO to rGO and formation of the Coffea arabica/rGO nanocomposite. The composite prepared from young coffee leave exhibited higher antioxidant capacity than matured leave against scavenging 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. Fascinatingly, the Coffea arabica/rGO nanocomposite showed an anti-inflammatory activity as well suggesting that the Coffea arabica /rGO nanocomposite is promising candidate for bio-medical applications in near future.
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