Rice, J.H. (2015) "Quantifying nanoscale biochemical heterogeneity in human epithelial cancer cells using combined AFM and PTIR absorption nanoimaging" Journal of Biophotonics, 8(1-2) : 133-141 which has been published in final form at http://dx
This paper reports on the use of infrared (IR) nanospectral absorption imaging to map the subcellular localization of toluidine blue-conjugated gold nanoparticles within colon adenocarcinoma cells. The spatial and spectral accuracy of the IR imaging method is confirmed via co-localization of the nanoparticles on a cell by cell basis using conventional fluorescence microscopy. IR spectral ratio imaging is presented as a means to map intracellular nanoparticle density at sub 50 nm lateral resolution with IR nanospectroscopy enabling distinction of nanoparticle seeded cells from a control group with 95% confidence. In this way we illustrate 2 that IR absorption nanoimaging, combined with IR point source data permits extension of the AFM-IR technique from subcellular analysis up to studies of cell numbers that are statistically significant.
Raman-spectroscopy-based methods, such as surface-enhanced Raman spectroscopy, are a well-evolved method to molecular fingerprint cell types. Here we demonstrate that surface-enhanced Raman spectroscopy can enable us to distinguish cell development stages of bone marrow hematopoietic stem cells towards red blood cells through the identification of specific surface-enhanced Raman spectroscopy biomarkers. The approach taken here is to allow cells to take in gold nanoparticles as Raman enhancement platforms for kinetic structural observations presented here through the view of the multidimensional parameter contribution, thereby enabling profiling of bone marrow hematopoietic stem cells acquired from proliferation (stage one), differentiation (stage two), and mature red blood cells (stage three).
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