Man-made microwave and radiofrequency (RF) radiation technologies have been steadily increasing with the growing demand of electronic appliances such as microwave oven and cell phones. These appliances affect biological systems by increasing free radicals, thus leading to oxidative damage. The aim of this study was to explore the effect of 2.45 GHz microwave radiation on histology and the level of lipid peroxide (LPO) in Wistar rats. Sixty-day-old male Wistar rats with 180 ± 10 g body weight were used for this study. Animals were divided into two groups: sham exposed (control) and microwave exposed. These animals were exposed for 2 h a day for 35 d to 2.45 GHz microwave radiation (power density, 0.2 mW/cm). The whole-body specific absorption rate (SAR) was estimated to be 0.14 W/kg. After completion of the exposure period, rats were sacrificed, and brain, liver, kidney, testis and spleen were stored/preserved for determination of LPO and histological parameters. Significantly high level of LPO was observed in the liver (p < 0.001), brain (p < 0.004) and spleen (p < 0.006) in samples from rats exposed to microwave radiation. Also histological changes were observed in the brain, liver, testis, kidney and spleen after whole-body microwave exposure, compared to the control group. Based on the results obtained in this study, we conclude that exposure to microwave radiation 2 h a day for 35 d can potentially cause histopathology and oxidative changes in Wistar rats. These results indicate possible implications of such exposure on human health.

10 GHz field has an injurious effect on fertility potential of male-exposed animals.

The radioprotective effect of Grewia asiatica fruit (GAE) which contains anthocyanin-type cyanidin 3-glucoside, vitamins C and A, minerals, carotenes and dietary fibre was studied. For the study Swiss albino mice were divided into five groups: (1) control (vehicle treated); (2) GAE treated (700 mg kg(-1) day(-1) for 15 days); (3) irradiated (5 Gy); (4) GAE+irradiated and (5) irradiated+GAE treated. The irradiation of animals resulted in a significant elevation of lipid peroxidation in terms of thiobarbituric acid reactive substances (TBARS) content and depletion in glutathione (GSH) and protein levels at all intervals studied, namely 1-30 days, in comparison to the control group. Treatment of mice with GAE before and after irradiation caused a significant depletion in TBARS content followed by a significant elevation in GSH and protein concentration in the intestine and testis of mice at all post-irradiation autopsy intervals in comparison to irradiated mice. Significant protection of DNA and RNA in testis was also noticed. GAE was found to have strong radical scavenging activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH(*)) and O(2)(-) assays and also showed in vitro radioprotective activity in protein carbonyl assay in a dose-dependent manner. The above results prove the radioprotective efficacy of GAE.

BackgroundReligious beliefs and practices have long influenced human perceptions and uses of nature. Animals in particular play a prominent role in magico-religious practices and provide historical and cultural depth of these relationships. Understanding human-faunal relations is often fundamental to the cause of meaningful wildlife conservation. This study investigates the domestic and wild harvested species used for spiritual and religious purposes among the tribals of six tehsils of Udaipur district.MethodsThe ethnozoological data were obtained by an emic approach, applying different tools such as semi-structured interviews, participatory rural appraisal, key informant interviews, and focus group discussions. The scientific name and species of animals were identified using relevant and standard literature. Present investigation is a part of major concept worked out for study on tribal people and their beliefs. Ethnozoological information was collected by interview of 150 tribals. The questionnaire was prepared in Hindi keeping all parameters in mind. A total of 55 respondents (35 males and 20 females) answered to the magico-religious parameter. The collected data were analyzed through informant fidelity level (FL).ResultsThe present study was undertaken to have an insight of the ethnozoological uses of animals prevalent in Bhil, Meena, and Kathodi tribes inhabiting the Udaipur district of Rajasthan. A total of 25 animals used for magico-religious and social purposes were recorded from the study area. Out of the total number of animals, 60% (15) were mammals, 24% (6) were birds, 12% (3) were reptiles, and the rest 4% (1) were the mollusks. Of the total ethnozoological practices, 64% fall in the magico-religious category, 12% in socio-cultural category, 12% in the category of ethnomusical, and 12% in the category of taboos.ConclusionsThe tribal people maintain strong ties with animals at both the material and spiritual level. Study reveals that traditional people depend on local therapies either magico-religious or natural ones in absence of awareness, modern medical facilities, expensive drugs, and poor transportation. However, the use of animal material in such practices is on a decline.

Reports of declining male fertility have renewed interest in assessing the role of environmental and occupational exposures to electromagnetic fields (EMFs) in the aetiology of human infertility. Testicular functions are particularly susceptible to electromagnetic fields. The aim of the present work was to investigate the effect of 10-GHz EMF on male albino rat's reproductive system and to investigate the possible causative factor for such effect of exposure. The study was carried out in two groups of 70-day old adult male albino rats: a sham-exposed and a 10-GHz-exposed group (2 h a day for 45 days). Immediately after completion of the exposure, animals were sacrificed and sperms were extracted from the cauda and caput part of testis for the analysis of MDA, melatonin, and creatine kinase. Creatine kinase results revealed an increased level of phosphorylation that converts creatine to creatine phosphate in sperms after EMF exposure. EMF exposure also reduced the level of melatonin and MDA. It is concluded that microwave exposure could adversely affect male fertility by reducing availability of the above parameters. These results are indications of deleterious effects of these radiations on reproductive pattern of male rats.