Background: Milk is one of the most used foodstuffs by humans in their diet. The quality of goat milk is already regulated by Law, which takes into consideration its physical-chemical and microbiological composition parameters. Several factors contribute for the alteration of the physical-chemical and microbiological parameters of caprine milk. These include breed, age, lactation phase and diet, among others. The health status of animals may interfere in the quality of the milk produced, both regarding food safety and milk processing and, for that reason, the identification of mastitis-causing agents is important, so that prevention and control measures can be implemented. The present study aimed at analyzing the occurrence of mastitis and the milk composition of dairy goats in a semi-intensive production system. Materials, Methods & Results:In a private goat farm with a flock composed of 32 female animals of breeds such as Saanen and Anglo-Nubian, raised on a semi-confined system, the diagnosis of mastitis was made through methods such as California Mastitis Test (CMT), Somatic Cells Count (SCC) and bacterial isolation. A total of 136 milk samples were analyzed, which were individualized per teat. Additionally, the study determined the volume of milk produced individually and the chemical composition of milk samples (fat, raw protein, lactose and total solids). In 37 (27.2%) samples, subclinical mastitis caused by CNS was identified. A negative response to CMT was observed in 125 (91.92%) samples. Only four samples presented a coincidence (OR=1.239) between CMT and bacterial isolation (P=0.7470). It was observed that the daily milk production ranged between 207.90 mL and 2.68 L. Moreover, it became clear that the milk production in half of the teats with subclinical mastitis was smaller (P=0.0026). A tendency to a significant reduction (p< 0.001) in the percentage of fat and total solids was verified. The lactose percentage remained stable during the four months, and that of protein showed a tendency to reduction (p<0.001). A great variability in SCC was observed (23,000 to 9,999,000 cel.mL -1 ) during the sampling months. A significant concordance (P<0.001) was determined between a low SCC and the non-bacterial isolation in 65 samples (OR=11.556). Discussion: Mastitis is an inflammation of the mammary gland and, in the majority of cases, it is manifested in its subclinical form, having CNS as its main agent, both in Brazil and in other countries. Methods such as bacterial isolation, SCC and CMT are used for the diagnosis of mastitis. In the present study, no relation among these diagnostic methods has been found. This fact had already been reported in Brazilian literature. The herd configuration may have contributed for the occurrence of mastitis, since this disease is influenced by genetic factors and age. Moreover, its occurrence was higher in Anglo-Nubian females which, in addition to being older and consequently having undergone a higher number of lactations, presented an udder conformation with fragile lig...
RESUMO O rebanho de ovinos no Brasil está estimado em mais de 16 milhões de cabeças. Embora o consumo da carne desta espécie ainda seja pequeno, comparado ao de outros países, o consumo de carne, inclusive ovina, tem sido associado às doenças transmitidas por alimentos, em especial a salmonelose. No presente estudo, investigou-se a ocorrência de salmonelas em linfonodos mesentéricos e conteúdo intestinal de 175 ovinos ao abate. “Pools” constituído por cinco amostras de contéudo fecal ou 5 amostras de linfonodos de 25 g foram pre-enriquecidos em 250 mL de água peptonada tamponada e incubados a 37° C por 18-24 horas. Uma alíquota de 0,1 mL do pré-enriquecimento foi transferida para 9,9 mL de caldo de enriquecimento Rappaport-Vassiliadis e 1,0 mL do pré-enriquecimento foi transferido para 10 mL de caldo tetrationato Muller-Kaufmann, incubados a 42° C for 24h. 10 μL do caldo de enriquecimento foi semeado superfície de placas de ágar BPLS e ágar XLT4 incubadas a 37º C for 24-48h. Colônias suspeitas de salmonela foram testadas por provas bioquímicas e serologicas. Os testes bioquímicos utilizados para identificação de Salmonella foram TSI (triple sugar iron àgar), LIA (lysine iron àgar) e ágar ureia. Sorotipagem foi realizada no Laboratório de Enterobactérias do Instituto Osvaldo Cruz. Isolou-se Salmonella Tiphymurium de um pool de linfonodos mesentéricos, provenientes de cinco animais. O fato de se observar a ocorrência de salmonela em ovino portador sadio alerta para necessidade de monitorar este micro-organismo também nesta espécie, especialmente quando destinada ao abate, com vistas à produção de alimentos seguros.
Timomas são neoplasias de timo, localizados no mediastino, sendo o segundo tumor mais frequente encontrado neste local, contudo é um tumor raro em cães. Essa neoplasia afeta cães idosos, não havendo predisposição por raça ou sexo. Timomas são classificados em benignos ou malignos dependendo do grau de invasão nas estruturas adjacentes, metástases, ocorrência de síndromes paraneoplásicas e na possibilidade de ressecção cirúrgica. O tratamento de eleição é a timectomia, porém existem também a quimioterapia e radioterapia, estas não possuem o objetivo de cura. O prognóstico é dependente do grau de malignidade do timoma. O objetivo é relatar um caso de um canino com um timoma maligno inoperável e que foi realizado quimioterapia para citorredução.
Sobrevivência de salmonella em sistema anaeróbio para o tratamento de dejtos suínos Survival of salmonella in anaerobic system for the treatment of pig waste
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