No abstract
A novel approach for signal enhancement of electrochemical biosensors by incorporating mechanical vibrations has been developed. We report the electrochemical study of the ferricyanide and ferrocyanide Fe CN 6 − 3 / − 4 a redox couple, at room temperature (∼25 °C), under the effect of mechanical vibrations of different frequencies applied to the sensor, for sensitivity enhancement. The experimental results showed a sensitivity enhancement of ∼332% (from 3.125 nA μ M − 1 to 13.5 nA μ M − 1 ) at 88.75 Hz of vibration. This novel approach of signal sensitivity enhancement is also validated with antibody immobilization-based Vi antigen detection for typhoid assessment. The sensitivity enhancement up to ∼15% is achieved for Vi antigen detection under the mechanical vibration of 120 Hz. The sensor is fabricated using microfabrication technology. The vibration-assisted ultrasensitive biosensing platform is also prototyped as a portable and Internet of Thing (IoT) enabled device, suitable for point-of-care applications. Detailed features of the prototype along with the test results are elaborated in the paper.
A highly sensitive and robust polysilicon Field Effect transistor (SiFET) has been developed for the detection of an gold standard biomarker of myocardial infarction (AMI) Cardiac Troponin I (cTnI). This paper reports a polysilicon FET device integrated with a thin film of gold layer as a gate terminal which is biofunctionalized with specific cTnI binding single stranded DNA receptor aptamer to detect cTnI. The reported SiFET is fabricated using standard microfabrication unit processes. The detailed characterization of reported device is performed and thoroughly discussed at different stages of device development. The assessment of bio-interface is also studied using various surface characterization techniques such as Raman spectroscopy, Atomic force microscopy (AFM) and Contact angle measurements. The experimental results showed the significant response for cTnI concentration as low as 0.1 ng ml−1 for label free detection.
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