Abstract. Hidayaturrahmah, Mabrur, Santoso HB, Sasmita R, Rahmy USA, Badruzsaufari. 2019. Short Communication: Protein profiles of Giant Mudskipper and its potential use as biomarker candidate for heavy metal contamination in Barito Estuary, Indonesia. Biodiversitas 20: 745-753. Giant mudskipper (Periphthalmodon schlosseri, Pallas 1770) is one of important biological diversity that potential as bio-indicator in environment assessment which is heavy metal contamination. These fish are susceptible to ambient environment, that effect to their physiological mechanism including protein synthesis and expression. This research aims to identify protein profiles of giant mudskipper muscle and review its potential application as a biomarker for heavy metal contamination in Barito Estuary of South Kalimantan. Total proteins were isolated from fish muscle using Tris EDTA buffer and then precipitated using Ammonium sulfate salt. The protein concentration was measured using Bradford assay and then separated based on molecular weight using the SDS-PAGE method. The result showed muscle proteins of giant mudskipper could be precipitated on optimum condition at Ammonium sulfate saturation 60-70% which protein concentration 5.106 mg.mL-1. The protein separated into 20 bands for raw and precipitated proteins which molecular weight ranging from 33 into 184 kDa. Further study is needed for protein identification based on amino acids sequence of protein bands for biomarker discovery and validation.
Abstract. Sasmita R, Mabrur, Rahmy USA, Badruzsaufari. 2019. Genetic diversity and phylogenetic relationship among Anabantoidei fish (Anabantiformes) in South Kalimantan, Indonesia based on SDS-PAGE analysis. Biodiversitas 20: 2519-2527. Protein profile is a molecular marker for diversity and phylogenetic analysis of germplasm, including Anabantoidei fish which is abundant in freshwater ecosystem of South Kalimantan. The aim of this research was to analyze genetic diversity and construct phylogenetic relationship among Anabantoidei fish based on SDS PAGE analysis. Protein was extracted from muscle tissue of six species of Anabantoidei fish and precipitated using Ammonium sulfate salt. Soluble protein content was determined using Bradford assay and then separated based on SDS-PAGE method. Genetic diversity and phylogenetic relationship were constructed using PAST software based on UPGMA method. The results showed that the soluble protein of fish muscle can be precipitated optimally in different ammonium sulfate concentration. Based on SDS-PAGE analysis, 59 different protein bands have been separated from gels with molecular weight ranging from 28.15 to 181.61 kDa. On the protein level, the Anabantoidei fish showed high genetic polymorphism (greater than 90%) with 3, 2 and 5 monomorphic bands on non-precipitated, AS-precipitated and combination between non- and AS-precipitated proteins, respectively. The phylogenetic reconstruction also exhibited that the Anabantoidei fish has the unique phylogenetic trees, especially for the combined protein datasets. This information would be useful for freshwater fish conservation and breeding programs.
This paper had described the protein profile of kihung or snakehead fish in Rawoijo Lake, the located east of the prehistorics sites, at Bukit Bangkai, South Kalimantan. There had not been many studies of the Kihung (Channa lucius) in this prehistoric sites. This information on protein profiles and weights had a strategic role in the management of the site area for educational and scientific tourism purposes. The analysis of kihung fish was carried out by describing morphology, morphometric and meristic calculations and comparison kihung fish from Banjar Regency, almost 400 Km from the Bukit Bangkai sites too.The protein content was measured by the Lowry method. Protein profiles were analyzed using the SDS-PAGE and elaborated using the UPGMA method. The results of morphological observations of kihung fish have a lighter body color and all fins are thick with regular patterns from Lake Rawoijo, while fish from Banjar Regency have a dark brownish body color. These fish have thin fins all with a pattern that is not irregular too. Optimum protein levels dissolved in kihung fish meat from Rawoijo, sample
The proboscis monkey's habitat is a mangrove area dominated by rambai (Sonneratia caseolaris) as their main food source. In order to suppress the rate of decline in the proboscis monkey population due to habitat loss, it is necessary to restore proboscis monkey habitat by planting rambai mangroves in their natural habitat areas, such as Curiak Island, to increase the carrying capacity of feed. So far, the rambai tree planting activity on Curiak Island is still being carried out on a self-taught basis by partners. So, there are still obstacles such as the low survival rate of seedlings, slow growth rates, challenges in transferring seedlings to planting sites, and pests and plant diseases that interfere with the growth of rambai mangroves. In this program, it has been conducted the introduction of Composter as a tool for processing organic waste into fertilizer to assist the seedling process, so that the survival of the seeds increases. Partners are also given training and counseling on the Rambai Mangrove Restoration technique with assistance starting from seed selection, transportation of seeds to planting sites, planting, and maintenance. A total of 2 composter barrels and 100 rambai mangrove seedlings have been planted with partners on Curiak Island. After this program, partners are judged to have been able to absorb the science and technology transferred by the team with an in the success of planting rambai mangroves at the planting location, Curiak Island. In future, partners are expected to independently carry out a sustainable rambai planting program so that efforts to restore proboscis monkey habitat and conserve river ecosystems can be realized.
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