laboratory experiment was conducted using the completely randomised design (CRD) to estimate CO2 released from the soil in one of the labs at the College of Agriculture and Forestry using two levels of fungus inoculant (without inoculation and inoculation with Trichoderma harzianum) with 10 cm3, and two levels of cow amendment (without manuring and 2% manure). This experiment was conducted in two types of soils, the first was taken from one of the fields at the College of Agriculture and Forestry and the second soil was taken from the Zaweetah area, which is planted with pine trees targeting the release of CO2 that is considered as an indicator of the biological decomposition of organic fertilisers. The experimental units were incubated for 10, 20, 40 and 60 days at a temperature of 28°C ±2. The results showed that inoculating the soil with T. harzianum fungus, in the presence of the organic amendment (cow manure), gave the highest value of CO2 release at the incubation period of 40 days for both soils as the CO2 release values were 52.9 and 57.7 mg.100 g–1 soil. Moreover, a decrease in the quantity of CO2 released was observed at the incubation period of 60 days in both soils.
The effect of four sources of organic manures (alfalfa residue, cow, chicken and sheep) and their concentrations (0, 1 and 2%) on the enzymatic activity of three calcareous soils from Northern Iraq have been studied in a 45-day incubation experiment at 28C and 90% of the field capacity. During this period, the activity of urease, asparginase and glutaminase have been measured at a 2-week intervals. Results indicated that the more the concentration of the added manure, the more the activity of the enzymes. Addition of alfalfa residue resulted in more activity, followed by chicken manure, then sheep. The least effect was obtained in soils treated with cow manure.
During the winter season 2021-2022, an experiment was carried out in the wooden canopy of the College of Agriculture and Forestry / University of Mosul to study of effect of Rhizobacterein, Nano-NPK20:20:20, and Nano-Micro Complete fertilizers and time periods on the number of rhizosphere bacteria and some of the growth and yield parameters of bean. Using (Silt Loam) texture in Alhawi soil in Mosul region. The first factor contained nine treatments of (NPK20:20:20), (Micro Complete) nano-fertilizer, Rhizobacterein bio-fertilizer, dual and tri combination of them, In addition to a treatment containing conventional fertilizers, as well as a control. The second factor is the time periods (60, 90 and 120) days of cultivation using a complete random design (CRD) with three replications. On 24/11/2021, the seeds of the Italian-origin bean Luz cultivar were planted in polyethylene (10) kg of soil. Fertilizers were combined with the soil and added in two batches, the first after germination and the second 20 days later. For each time, the total number of bacteria and vegetative properties were counted. (60, 90, and 120) cultivation days. The results of the statistical analysis for the least significant difference (L.S.D0.05) showed superiority of the tri combination of fertilization (Nano-NPK+Nano-Micro complete+Rhzobacterein) for the period (120) days after planting in the chlorophyll index, fresh pods yield, and fresh plants yield (51.84 SPAD, 71.62 g plant−1, and 33.85 g plant−1) respectively, compared to the same combination of the traditional sources (49.2 SPAD, 62.86 g plant−1, and 24.72 g plant−1) for the same period, while the same trend was achieved with the total numbers of bacteria for the tri nano, bio fertilizers combination (6.93, 10.12, 5.03) (108) cfu g−1soil, compared to the tri traditional, bio fertilizers combination (5.14, 6.73, 4.59) (108) cfu g−1 soil, respectively for the periods (60, 90, and 120) days after of planting.
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