Bacterial vaginosis is a vaginal condition characterized by an abnormal vaginal discharge due to an overgrowth of normal bacteria in the vagina. Women with bacterial vaginosis also have fewer than the usual population of vaginal bacteria, i.e lactobacilli. In this study vaginal samples from 75 women were taken to detect and quantify two putative positive-indicator of bacterial vaginosis namely, Atopobium vaginae and Megasphaera type1, in addition to a negative-indicator of bacterial vaginosis (Lactobacillus crispatus) using real-time polymerase chain reaction technique. The prevalence of bacterial vaginosis was 34.66% according to scoring system, the most dominant species in patients and in women without the syndrome was L. crispatus (80.76 and 79.59% respectively), but its concentration of 16S rRNA genes was significantly higher in bacterial vaginosis negative women (2.8×108 vs. 4.74×107 copy/swab). In bacterial vaginosis patients, the highest rDNA concentration was for Megasphaera1 (3.10×108 16S rRNA copy/swab).
Septicemia is a serious bloodstream infection; it can quickly become life-threatening. The current study aimed to isolate and identify the causative agents of septicemia cases with reference to the antibiogram test. 52 blood samples were collected from a number of incomings and those who lie in Hilla general hospital. After incubation and culturing on suitable media, 30 samples gave growth of one or two species of bacteria. A total of 33 bacterial isolates were obtained, most of them (16 isolates) were belong to coagulase-negative Staphylococcus spp., followed by E. coli (6 isolates), Pseudomonas sp. (5 isolates), Streptococcus sp. (2 isolates), and one isolate for each of Staphylococcus aureus, Streptococcus pneumonia, Acinetobacter sp., and Listeria monocytogenes. When antibiotics sensitivity test was accomplished, most Staphylococcus spp. were sensitive for daptomycin and had high resistance to both of vancomycin and clindamycin, whereas Staphylococcus aureus was sensitive for most the used antibiotics. Half of E. coli isolates were sensitive, while the second half were resistant to the used antibiotics. Imipenem inhibited the growth of all Pseudomonas isolates, whereas 80% of them were resistant to amikacin. The epidemiology of bacteremia is altering with the aging of the population, shifts in healthcare, and progress in medicine, such as increased use of immunosuppressive treatment, intravascular devices, and invasive procedures.
In this work, zinc oxide nanoparticles (ZnO - NPs) were prepared using a sol-gel methodology and tested for their antibacterial activity against each of the following pathogenic species: Escherichia coli, Klebsiella pneumonia, and Staphylococcus aureus by well diffusion assay. The sample prepared was characterized by different techniques: Atomic Force Microscope AFM; Fourier Transform Infrared FT-IR; Scanning Electron Microscope SEM and X-Ray Diffraction Spectroscopy XRD. The XRD result showed that ZnO - NPs presence in wurtzite the structure of ZnO. The AFM and SEM of the surface analysis indicate that the most ZnO – NPs appear approximately in a spherical shape with some agglomeration. The average particle size for ZnO - NPs is nearly 37 nm. Volumes 25µl, 50µl, 75µl, 100µl, 125µl, and 150µl of 10 mg\ ml concentration of ZnO - NPs were used, the antimicrobial activity results showed that ability for ZnO - NPs to inhibit the growth of S.aureus increased as the solution volume increased, while the growing of (K. pneumonia) and (E. coli) was inhibited only with the volume 75µl where the inhibition zones diameters were 15mm and 10mm respectively.
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