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The seroprevalence of human herpesvirus 8 (HHV-8) was studied in 326 human immunodeficiency virus (HIV)-positive and -negative persons from Honduras; women constituted 77% (n = 251) of the subjects. Sera were tested for lytic HHV-8 antibodies by an IFA, and positive samples were confirmed by a radioimmunoprecipitation assay. Of the 326 persons tested, 58 (17.8%) had HHV-8 antibodies. Among the HIV-infected women, 22.7% were seropositive; 11.3% of the HIV-negative women were seropositive. HHV-8 seroprevalence was almost four times higher in HIV-positive female commercial sex workers (36%) than in HIV-negative female non-commercial sex workers (9.9%; odds ratio = 3.8, 95% confidence interval = 1.1-13; P = 0.01), suggesting that commercial sex work is a risk factor for HHV-8 infection. In the men studied, the overall HHV-8 seroprevalence was 22.6%, with a seropositivity rate of 28% for HIV-positive men compared with 12% for HIV-negative men.

Purpose Zika virus (ZIKV) statistics in Honduras are the highest among countries in Central America. National risk communication strategies have primarily focused on vector control and are integrated into existing approaches for Dengue and Chikungunya. Given the new evidence on ZIKV, there is a need to revamp risk communication strategies so that they are informed by dynamic listening methods such as knowledge, attitudes, and practices. The paper aims to discuss this issue. Design/methodology/approach A cross-sectional survey was administered to 604 respondents in 21 designated Honduran communities using a two-stage, 30-cluster sampling method. Findings Almost eight out of ten Hondurans knew that Zika can be transmitted by the bite of a mosquito; however, only 2 and 0.1 percent, respectively, were aware that Zika can be spread by sexual intercourse and from a pregnant woman to her fetus. In total, four out of ten Hondurans knew that there is a causal relationship between Zika and microcephaly in newborns, and three out of ten knew that there is an association between Zika and Guillian-Barré syndrome. Overall, 50 percent of respondents said that they did not have enough information about the disease. Social implications The findings of this study clearly identify information priority gaps that need to be urgently addressed by national stakeholders involved in public health activities to protect the most vulnerable population against Zika disease and its complications. Originality/value This study is the first of its kind in Central America to inform any national risk communication strategy since the inception of the ZIKV response, particularly among at risk populations.

Proper sampling is essential to characterize the molecular epidemiology of human immunodeficiency virus (HIV). HIV sampling frames are difficult to identify, so most studies use convenience samples. We discuss statistically valid and feasible sampling techniques that overcome some of the potential for bias due to convenience sampling and ensure better representation of the study population. We employ a sampling design called stratified cluster sampling. This first divides the population into geographical and/or social strata. Within each stratum, a population of clusters is chosen from groups, locations, or facilities where HIV-positive individuals might be found. Some clusters are randomly selected within strata and individuals are randomly selected within clusters. Variation and cost help determine the number of clusters and the number of individuals within clusters that are to be sampled. We illustrate the approach through a study designed to survey the heterogeneity of subtype B strains in Honduras.