Introduction: Free radicals increase with age and disease, so the aim of this study was to investigate the effect of exercise and vitamin D on the expression of alkaline phosphatase and osteocalcin genes in bone tissue of rats poisoned with hydrogen peroxide. Methods: In this experimental trial, 36 adult male Wistar rats were randomized into six groups of six rats, 1) control; 2) hydrogen peroxide; 3) hydrogen peroxide + vitamin D; 4) hydrogen peroxide + exercise; 5) hydrogen peroxide + exercise and vitamin D and 6) Sham. For eight weeks, groups 2, 3, 4, and 5 were given daily dose of 1 mmol/kg hydrogen peroxide on even days, groups 3 and 5 received 0.5 mg / kg of Vitamin-D daily, and sham group received only vitamin D solvent intraperitoneally. Groups 4 and 5 performed aerobic exercise 3 day/week. Osteocalcin and alkaline phosphatase gene expression were measured by PCR and were analyzed using independent t-test, two-way analysis of variance and Boferroni’s post hoc test with SPSS 16 (p≤0.05). Results: The interactive effect of exercise and vitamin D on increasing alkaline phosphatase and osteocalcin was significant. (p≤0.05); exercise increased alkaline phosphatase and osteocalcin (p ≤ 0.05); vitamin D was also associated with increased alkaline phosphatase and osteocalcin (p=0.0001). The greatest effect on increasing osteocalcin and alkaline phosphatase showed in groups 5 and 3, respectively (p=0.001). Conclusion: Exercise and vitamin D had a positive effect on bone tissue, so that even the systemic effect of hydrogen peroxide could not change the results of this constructive effect.
Background: Exercise and vitamin D can improve bone density by reducing bone loss. Growth factors such as IGF-I and IGFBP-3 are appeared to increase bone turnover in response to mechanical load, and free radicals attenuate the release of these growth factors. Objectives: We assessed the effect of concurrent aerobic training and cholecalciferol administration along with hydrogen peroxide injection on the levels of IGF-I and IGFBP-3 expression in the bone tissue of rats. Methods: Thirty-six adult Wistar rats were randomized into six groups (n = 6), including healthy control, sham, hydrogen peroxide (H2O2), H2O2 + aerobic training, H2O2 + cholecalciferol, and H2O2 + aerobic training + cholecalciferol. The rats were intraperitoneally administered with one mmol/kg. Body weight (BW) of H2O2 three times a week on even days and 0.5 μg/kg.bw of cholecalciferol daily. Aerobic training (at a speed of 4 - 20 m/min, for 20 - 60 minutes) was performed five days/w for eight weeks. The expression of IGF-I and IGFBP-3 was measured by real-time (RT)-PCR. Data were analyzed using the independent t-test, two-way ANOVA (exercise × vitamin D), and Bonferroni’s post-hoc test in SPSS 26 at the significance level of P ≤ 0.05. Results: The results showed that H2O2 significantly reduced the gene expressions of IGF-I (P = 0.001) and IGFBP-3 (P = 0.001) in the bone tissue. Also, exercise and vitamin D augmented the expression of IGF-I (P = 0.008) and IGFBP-3 (P = 0.0001) as post-hoc analysis showed that aerobic training had the greatest effect on the expression of IGF-I and IGFBP-3 (P < 0.05). In addition, the amplifying effects of aerobic training and cholecalciferol on the gene expressions of IGF-I and IGFBP-3 were also remarkable (P < 0.1). Conclusions: The mechanical load created by aerobic training exerted the greatest augmenting effect on the gene expression of IGF-I and IGFBP-3. Moreover, the interactive effect of aerobic training and cholecalciferol was also significant.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.