Coxiella burneti phase I, purified from a formalin-inactivated yolk-sac vaccine, was separated into two bands of morphologically distinct cell types when subjected to sucrose gradient centrifugation. Recycling of the less dense, rod-shaped cells in unbuffered sucrose gradients (pH 5.5 to 6.0) resulted in the formation of bands having the location and appearance of the original two bands. Recycling of the denser band of larger ovoid-shaped cells yielded a single band, suggesting that the larger cell type arose from the smaller cell. In contrast to vaccine-derived rickettsiae, live, cell culture-propagated phase I organisms formed a single band in unbuffered sucrose gradients, at the same density as the upper band of the vaccine preparation. Centrifugation of cell culture-derived rickettsiae for 26 to 48 h in sucrose gradients of pH 5.5 resulted in the formation of a second band, at the same density as the lower band of the vaccine preparation. This did not occur in gradients of pH 7.0. Treatment of cell culture-propagated rickettsiae with formalin or germicidal ultraviolet radiation induced a total shift of the less dense cell population to a zone of higher density when centrifuged isopycnically in CsCl gradients. This density change did not occur in sucrose gradients, suggesting a difference in the effect of these treatments on the permeability of the cell membrane to sucrose and CsCl.
Purpose -The purpose of this paper is to study cyber-enabled manufacturing systems (CeMS) for additive manufacturing (AM). The technology of AM or solid free-form fabrication has received considerable attention in recent years. Several public and private interests are exploring AM to find solutions to manufacturing problems and to create new opportunities. For AM to be commercially accepted, it must make products reliably and predictably. AM processes must achieve consistency and be reproducible. Design/methodology/approach -An approach we have taken is to foster a basic research program in CeMS for AM. The long-range goal of the program is to achieve the level of control over AM processes for industrial acceptance and wide-use of the technology. This program will develop measurement, sensing, manipulation and process control models and algorithms for AM by harnessing principles underpinning cyber-physical systems (CPS) and fundamentals of physical processes. Findings -This paper describes the challenges facing AM and the goals of the CeMS program to meet them. It also presents preliminary results of studies in thermal modeling and process models. Originality/value -The development of CeMS concepts for AM should address issues such as part quality and process dependability, which are key for successful application of this disruptive rapid manufacturing technology.
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