Significance and Impact of the Study: White-rot fungus, Phlebia brevispora was evaluated for its heavy metal removal efficiency. Phlebia brevispora significantly removed all the metals from the solution; however, the metal removal efficiency varied with the type of metal ion. It seems that the possible metal removal mechanisms involved intracellular compartmentation of metals in vacuoles.
The study was designed to evaluate the production of auxin by eukaryotic unicellular organism Pichia fermentans. Different media formulations were used for the production of indole-3-acetic acid (IAA) under broth and submerged conditions. Wheat straw-based production medium was formulated and optimized using statistical approach. The IAA production was significantly enhanced by nine folds, when the wheat straw was pretreated with Phanerochaete chrysosporium (150 µg/ml) as compared to untreated wheat straw (16.44 µg/ml). Partial purification of IAA was carried out by silica gel column chromatography and further confirmed by high-performance liquid chromatography. Exogenous application of crude and partially purified IAA positively influenced the Vigna radiata seedling growth. The number of lateral roots in the growing seedlings was significantly higher as compared to the control seeds. Thus, the present findings point towards an efficient production of plant hormone by yeast and white rot fungus using abundantly available wheat straw, which may lead to the development of cost-effective production of such metabolites and their further use in agricultural field to reduce the negative impact of chemical fertilizers.
The coronavirus disease (COVID‐19) has been identified as a pandemic and affected almost whole world. Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), the causative agent of this disease, is infecting Indian population since the last week of January 2020. The data were collected from January 30, 2020, to May 23, 2020, to analyze basic trend of COVID‐19 cases in India targeting hotspot regions. To find the linear relationship between variables, that is, age, total positive cases, population, and population density data have been statistically analyzed. COVID‐19 caused more than 5000 deaths till May 2020 in India. SARS‐CoV‐2 spread to several Indian cities with more than 100,000 positive cases. Total number of COVID‐19 cases and total recovered cases followed the exponential distribution, while number of deaths showed linear behavior. Nearly 50% of the youth, that is, 20–40 years of age had been found to recover from the infection. As a lockdown cannot be a permanent solution, it is important to understand the nature of virus and learn “living with the virus” while minimizing its spreading at the same time.
Auxin is a plant hormone, which mainly includes indole acetic acid (IAA). The study was designed to evaluate and compare different techniques used to check the purity of IAA produced by Phanerochaete chrysosporium. Colorimetric method was used for the qualitative and quantitative determination of crude IAA. Partial purification of IAA was carried out by silica gel column chromatography. UV-Visible spectrophotometric analysis revealed the high similarity of the purified compound with standard IAA. Besides having a comparable UV-Visible spectrum, high performance liquid chromatography (HPLC) analysis showed two peaks, which also confirmed the presence of IAA. Thus the article describes efficient production of IAA by Phanerochaete chrysosporium and explores laboratory level experiment design to produce such bioactive compound with different methods to check its purity, which is simple, accurate and rapid to be employed for screening of new compounds.
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