Production of Indole Acetic Acid by a Wood Degrading Fungus Phanerochaete chrysosporium

Abstract

Auxin is a plant hormone, which mainly includes indole acetic acid (IAA). The study was designed to evaluate and compare different techniques used to check the purity of IAA produced by Phanerochaete chrysosporium. Colorimetric method was used for the qualitative and quantitative determination of crude IAA. Partial purification of IAA was carried out by silica gel column chromatography. UV-Visible spectrophotometric analysis revealed the high similarity of the purified compound with standard IAA. Besides having a comparable UV-Visible spectrum, high performance liquid chromatography (HPLC) analysis showed two peaks, which also confirmed the presence of IAA. Thus the article describes efficient production of IAA by Phanerochaete chrysosporium and explores laboratory level experiment design to produce such bioactive compound with different methods to check its purity, which is simple, accurate and rapid to be employed for screening of new compounds.

Agricultural Bioinputs Obtained by Solid-State Fermentation: From Production in Biorefineries to Sustainable Agriculture

“…In this regard, the selective degradation of lignin to carbon dioxide can be carried out using white rot fungi. For instance, Giri and Sharma [194] suggested pretreating wheat straw using Phanerochaete chrysosporium, known for its ability to degrade lignin, as an alternative to accessing cellulose and converting it into easily fermentable sugars. Nevertheless, during secondary fermentation, these precursors may contribute to the synthesis of IAA via this fungus, along with the supplementation of tryptophan.…”

Assessment toxic effects of exposure to 3-indoleacetic acid via hemato-biochemical, hormonal, and histopathological screening in rats

“…The IAA in the samples detected by HPLC under the following conditions: Flow rate 1/min with isocratic elution, the injection volume was 50 μl of sample, and the detection wavelength was set at 254 nm. Mobile phase: 0.2% glacial acetic acid: methanol 50%:50%, retention time is 4.6 min and run time is 7 min (Giri et al 2020). Laboratory reagents were of analytical and HPLC grade and purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA).…”

“…It can be produced by microorganisms also, such as bacteria and fungi (Fu et al 2015), and its biosynthetic pathways in plants and bacteria are mainly similar, where both tryptophan (Trp)-dependent and Trp-independent IAA biosynthetic pathways occur in both of them (Mano and Nemoto 2012). The IAA molecular formula is C 10 H 9 NO 2 , and its chemical structure is a monocarboxylic acid and one of the methyl hydrogens has been replaced by a 1H-indol-3-yl group (Giri et al 2020). It is used commercially in wide forms, either dispersed in talc or in concentrated liquid compounds that can be diluted with water to induce more rapid plant growth, delay fruit drop, enhance root formation, and produce seedless varieties by parthenogenetic fruiting (Khan et al 2007;Serrani et al 2008;Moustakime et al 2017).…”