Summary BACKGROUND The intrinsic neural plexus of the mouse heart has not been adequately investigated despite the extensive use of this species in experimental cardiology. OBJECTIVE We determined the distribution of cholinergic, adrenergic and sensory neural components in whole-mount mouse heart preparations using double immunohistochemical labeling. METHODS AND RESULTS Intrinsic neurons were concentrated within 19±3 ganglia (n = 20 mice) of varying size, scattered on the medial side of the inferior caval (caudal) vein on the right atrium and close to the pulmonary veins on the left atrium. Of a total of 1082±160 neurons, most somata (83%) were choline acetyltransferase (ChAT)-immunoreactive, while 4% were tyrosine hydroxylase (TH)-immunoreactive; 14% of ganglionic cells were biphenotypic for ChAT and TH. The most intense ChAT staining was observed in axonal varicosities. ChAT was evident in nerve fibers interconnecting intrinsic ganglia. Both ChAT and TH immunoreactivity were abundant within the nerves accessing the heart. However, epicardial TH-immunoreactive nerve fibers were predominant on the dorsal and ventral left atrium, whereas most ChAT-positive axons proceeded on the heart base toward the large intrinsic ganglia and on the epicardium of the root of the right cranial vein. Substance P-positive and calcitonin gene-related peptide-immunoreactive nerve fibers were abundant on the epicardium and within ganglia adjacent to the heart hilum. Small intensely fluorescent cells were grouped into clusters of 3–8 and dispersed within large ganglia or separately on the atrial and ventricular walls. CONCLUSIONS While some nerves and neuronal bundles of the mouse epicardial plexus are mixed, most express either adrenergic or cholinergic markers. Therefore, selective stimulation and/or ablation of the functionally distinct intrinsic neural pathways should allow the study of specific effects on cardiac function.

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Despite a number of studies in the field of job satisfaction, there are still some problematic areas left. A question, which still lacks explanation, is whether specific sample may be evaluated using instruments which were primarily developed based on a different type of a sample than the one under research. With regard to the problematic area, we generated the purpose for our research.The purpose of the present study is to examine Job Satisfaction Survey's (JSS) relevance for estimation of job satisfaction in teacher population.Results of the standard Confirmatory Factor Analysis of the teachers' sample did not support the existence of 9 facets, suggesting that some of the JSS's scales do not reflect teachers' job satisfaction. The best model in the present study was determined to be a three facet model, including promotion, supervision and nature of work. The obtained research results, limitations and recommendations are discussed.

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BackgroundGanglionated plexuses (GP) are terminal parts of cardiac autonomous nervous system (ANS). Radiofrequency ablation (RFA) for atrial fibrillation (AF) possibly affects GP. Changes in heart rate variability (HRV) after RFA can reflect ANS modulation.MethodsEpicardial RFA of GP on the left atrium (LA) was performed under the general anesthesia in 15 mature Romanov sheep. HRV was used to assess the alterations in autonomic regulation of the heart. A 24 − hour ECG monitoring was performed before the ablation, 2 days after it and at each of the 12 following months. Ablation sites were evaluated histologically.ResultsThere was an instant change in HRV parameters after the ablation. A standard deviation of all intervals between normal QRS (SDNN), a square root of the mean of the squared differences between successive normal QRS intervals (RMSSD) along with HRV triangular index (TI), low frequency (LF) power and high frequency (HF) power decreased, while LF/HF ratio increased. Both the SDNN, LF power and the HF power changes persisted throughout the 12 − month follow − up. Significant decrease in RMSSD persisted only for 3 months, HRV TI for 6 months and increase in LF/HF ratio for 7 months of the follow − up. Afterwards these three parameters were not different from the preprocedural values.ConclusionsEpicardial RFA of GP’s on the ovine left atrium has lasting effect on the main HRV parameters (SDNN, HF power and LF power). The normalization of RMSSD, HRV TI and LF/HF suggests that HRV after epicardial RFA of GPs on the left atrium might restore over time.

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Key competences underlying lifelong learning are particularly important in the knowledge society as they may guarantee more flexibility in the labor market and better adjustment to the permanent change. Entrepreneurial competence is one of the fundamental components for human employability and self-employment, often in conjunction with the ability to create and maintain jobs for themselves and others. Development of key skills and competences, as well as entrepreneurial competence, presents by itself potentially effective measure in coping with the existing skills mismatches, which is not sufficiently analyzed and considered in the current active labor market policies. The main research question: What is the role of the development of entrepreneurial competence in striving for employability and successful career? The aim of this paper is to identify the importance of entrepreneurial competence in job related activities: job search, keeping the job place, professional career. The object of the paperentrepreneurial competence of adults seeking employability and successful career.

Background:The data of various studies indicates, that stressful life events having a strong influence to the development of depression in adolescence.Aim:To study the correlation between psychosocial stress and depression among adolescents.Methods:The subjects of the study were 1026 schoolchildren (519 boys and 507 girls) of secondary schools (ninth form pupils) of one administrative region of Lithuania. Average age of adolescents was 14,8±1,12 years. The 57-item Depression scale of Minnesota Multiphasic Personality Inventory for Adolescents (MMPI-A D) and 7-item Reeder scale were used.Results:The first four factors of depression (pessimism, hostility, somatic health, tension-anxiety) after MMPI-A D scale orthogonal varimax rotation were used in data analysis. Correlative analysis was show statistically meaning correlations between depression, the pessimism factor and the all items of psychosocial stress for the girls. The boys’ tension-anxiety factor correlated statistically meaning with all items of psychosocial stress. Comparing depressive and not depressive adolescents according Reeder scale, determined that both: depressive boys (p=0,043), and depressive girls (p< 0,0001) experienced more psychosocial stress comparing with not depressive adolescents. The stepwise regression analyses differed an importance of the items “nervous tension” (p=0,011), “stress because of communication” (p=0,014) for the girls’ depression and the items “nervous tension” (p=0,011), “physical and mental exhaustion at the end of a day” (p=0,003) for the boys’ depression.Conclusions:1.Depressive boys and girls experienced more psychosocial stress comparing with not depressive adolescents.2.Psychosocial stress is important for prognosis of depression in adolescence.