The present study is designed to evaluate two sutures pattern techniques (inverting and appositional) for urinary bladder closure following experimental cystotomy in dogs. Thirty two adult local breed males’ dogs were enrolled for this study, aged 1-3 years and weighing 18-23 kg. Dogs were randomly allocated into two equal groups. In the first group, cystotomies were closed via double-layers of continuous inverting suture pattern (Lambert and Cushing); while, in the second group, bladders incisions were closed by single-layer appositional suture pattern (simple interrupted) which did not involve the mucosa (extra-mucosal). Synthetic absorbable suture material polydiaxnone PDS 3/0 was used to close the bladder in all experimental animal groups. Surgery was done under the effect of general anesthesia using a combination of ketamine at a dose rate of 15mg/kg and xylazine at a dose 5mg/kg B.W. Dogs were premedicated by atropine sluphate at a dose 0.03mg/kg. All drugs administered intramuscularly. There were highly significant (P <0.05) differences in operative times between the two groups. In inverting group, the time was 40 ± 4.50 minutes which was higher than that recorded for appositional group 25±2.50 minutes. The animals were followedup clinically during the studied period to record the secondary complications. Results reflected hematuria n=4, swelling of the operative site n=3 and urinary incontinence n=2. These complications were transient and disappeared in a short time. Also macroscopical and microscopical examinations were performed at 3,7,14 and 21 days post-surgery. Four dogs were used for each period. The main macroscopical finding was slight to moderate adhesions n = 3 between omentum and the wall of urinary bladder in both group. Microscopical examination of urinary bladder reflected earlier urolithium formation in appositional pattern (14 days) while inverted group (21 days). In conclusion, appositional pattern is technically easier and economic. Bladder regeneration was accomplished within 14 days in comparison with inverting pattern which reflected bladder regeneration at 21 days post-surgery.
The present study is assigned to evaluate the efficiency of hernioplasty in reconstruction of experimentally induced ventro-lateral hernia in bucks using two types of sutures (silk and polypropylene) and to find which thread is more safe and suitable for closure of the hernia, based on ultrasonographic examination.Sixteen adult local breed bucks were enrolled for this study. All subjected to inducing artificial ventro-lateral hernia (12x10) cm in the right flank under the effect of local anesthesia (2% Lidocaine), then left for one month. After that, animals divided randomly and equally into two groups. In the first group, hernia repaired with silk, while in the second group, hernia closed by polypropylene. Sewing technique is used in both groups. The final appearance was (mesh-like).Ultrasonographic examination in silk group and after 2 months there was slight improvement in echogenecity and collagen fibers. Polypropylene group and at the same period revealed an increased in echogenecity and decreased lesion size earlier in compare with silk group. In 4 months polypropylene group, reflected high echogenecity and complete infilling of the sewing holes with mature collagen fibers.
The aim of the current study was to compare the efficacy of Kessler suture pattern and polypropylene meshes implantation to repair severed Achilles tendons in bucks. For this purpos 16 local adult bucks, weighing 30-35kg were used and equally distributed into two groups, the first group: (control group) and the second group (mesh group). Animals were sedated with xylazine 2% and anesthetized locally with lidocaine hydrochloride 2% infiltrated subcutaneously. Skin incision approximately 5 cm. in length was made over the Achilles tendon. The tendon was isolated by blunt dissection from the underlying tissue, then the left Achilles tendon was transected in its mid portion. In the control group, tenorrhaphy of Achilles tendons were immediately performed by using Kessler suture technique using (polypropylene No. 1). In the second group, a polypropylene mesh was wrapped around the cut ends and fixed to the tendon by simple interrupted stitches of polypropylene thread (No.1). Then skin was sutured by interrupted horizontal mattress using silk No.1. Finally plaster of Paris with window was applied. The skin stitches were removed after 10 days. The clinical signs of all animals showed severe lameness during the first three weeks with no significant differences between the two groups. Lameness reflected significant differences (P<0.05) between groups with the progress of postoperative duration (i.e., starting from 4th week). Rapid absence of lameness was more observed in second group (at 4th week) than in first group (at 6th week). At two months post-operative, a higher percentage (100%) was recorded in first group. While a lower percentage (25%) was showed in the second group. Microscopical examination at two month post-suturing revealed proliferation of fibrous connective tissue around suture materials infiltrated by inflammatory cells, in addition to necrotic tissue attachment to the tendon. In mesh implantation and at the same time there were granulations tissues surrounding the narrow mesh holes with tendon fibers expressed proliferation of tenocytes. At four months, first group revealed few blood vessels, and thickened collagen fibers with mononuclear cells infiltration in cut tendon fibers. In mesh implantation the tendon was retained to its nearly normal structure with few (MNCs) in epitenon. It seemed that both groups gave best outcome in healing of operated tendons with superiority of the second group in comparison with the first group.
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